| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 496,170 | A→G | 45.9% | E609E (GAA→GAG) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 496,170 | 0 | A | G | 45.9% | 3.5 / 49.9 | 37 | E609E (GAA→GAG) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base A (8/12); new base G (10/7); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAT > W3110S.gb/496109‑496231 | tCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGa < 1:1319403/69‑1 (MQ=255) cGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCt < 1:436620/69‑1 (MQ=255) aGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGt < 1:3446632/69‑1 (MQ=255) aGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGt < 1:739962/69‑1 (MQ=255) gggTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTa < 1:1746918/69‑1 (MQ=255) ggTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTAt > 1:1129735/1‑69 (MQ=255) ggTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTAt > 1:795753/1‑69 (MQ=255) tAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTg > 1:2651062/1‑62 (MQ=255) aGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATtcg > 1:3610063/1‑69 (MQ=255) aGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATtcg > 1:1364814/1‑69 (MQ=255) gctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGa > 1:899909/1‑68 (MQ=255) gctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGa > 1:2126015/1‑68 (MQ=255) ggATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAAcc < 1:1055616/68‑1 (MQ=255) ggATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCa > 1:3222319/1‑69 (MQ=255) cAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAgctg > 1:847545/1‑69 (MQ=255) cAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAgctg > 1:878092/1‑69 (MQ=255) gctgctGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATg < 1:2351629/55‑1 (MQ=255) gctgctGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATg < 1:1090640/55‑1 (MQ=255) ctgctgGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATg < 1:652569/54‑1 (MQ=255) ggCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTg < 1:3443583/69‑1 (MQ=255) aGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGttt > 1:1934054/1‑62 (MQ=255) aGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGttt > 1:3436925/1‑62 (MQ=255) gAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:2618250/69‑1 (MQ=255) gAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:926043/69‑1 (MQ=255) gAACGCGGCACCCTGGAGGATCCGAAGCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTg < 1:3544946/65‑1 (MQ=255) aaCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:1791419/68‑1 (MQ=255) aCGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTaa > 1:2196488/1‑69 (MQ=255) aCGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTaa > 1:2431733/1‑69 (MQ=255) cgcgGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATg > 1:860704/1‑42 (MQ=255) cgcgGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAAt > 1:3307261/1‑69 (MQ=255) cgcgGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAAt < 1:445213/69‑1 (MQ=255) cgcgGCACGCTGGAAGATCCGAACCTGTTTAATCGTCGTATg < 1:3008469/42‑1 (MQ=255) cACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTa > 1:1015730/1‑62 (MQ=255) aCGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGa > 1:3089319/1‑37 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAt < 1:245283/69‑1 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAt < 1:1668190/69‑1 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAt < 1:1140377/69‑1 (MQ=255) | TCAGCGAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAT > W3110S.gb/496109‑496231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |