| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 505,179 | C→T | 34.6% | L348L (CTG→TTG) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 505,179 | 0 | C | T | 34.6% | 59.7 / 47.4 | 52 | L348L (CTG→TTG) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base C (22/12); new base T (12/6); total (34/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGT > W3110S.gb/505111‑505244 | gggAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGc < 1:1543444/69‑1 (MQ=255)gggAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGc > 1:3040989/1‑69 (MQ=255) ggAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCt > 1:1301540/1‑69 (MQ=255) aGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGtt < 1:1925370/69‑1 (MQ=255) aGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGtt < 1:1822575/69‑1 (MQ=255) gACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGc > 1:496256/1‑64 (MQ=255) gAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCAcc > 1:2829540/1‑69 (MQ=255) gagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGtt > 1:784765/1‑56 (MQ=255) gagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGtt < 1:162327/56‑1 (MQ=255) ttACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATGAc < 1:305918/50‑1 (MQ=255) tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCAc < 1:2814126/49‑1 (MQ=255) cacTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg > 1:200506/1‑68 (MQ=255) cacTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg > 1:3408348/1‑68 (MQ=255) cacTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAg > 1:2121672/1‑68 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAgcgc > 1:1584443/1‑69 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAgcgc > 1:1202953/1‑69 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAgcgc > 1:2528656/1‑69 (MQ=255) tCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCaa < 1:1832932/63‑1 (MQ=255) tCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCa > 1:61088/1‑69 (MQ=255) tCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCa > 1:125444/1‑69 (MQ=255) ccTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGtt < 1:3257120/38‑1 (MQ=255) ccTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACTGTTCCAGAACAAAGGCAAAGCGCAg > 1:1421642/1‑69 (MQ=255) ccTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg > 1:832666/1‑69 (MQ=255) cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGc > 1:3087641/1‑69 (MQ=255) aaaTCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg < 1:1459016/65‑1 (MQ=255) aaaTCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt > 1:2840140/1‑67 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACa < 1:2349735/49‑1 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACa > 1:570732/1‑49 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACa > 1:1982671/1‑49 (MQ=255) aTCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCa > 1:1561716/1‑55 (MQ=255) tCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg > 1:2324579/1‑66 (MQ=255) cGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt < 1:1217615/69‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGaa > 1:3334260/1‑44 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACaa > 1:2413751/1‑47 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1684807/69‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:3368135/69‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2060133/69‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:3665319/69‑1 (MQ=255) tGAAAACCAGCAAATGATCTCGCTGTTATCACCATTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2631602/69‑1 (MQ=255) ccAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAg > 1:1519447/1‑68 (MQ=255) tGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCa < 1:1646748/69‑1 (MQ=255) tGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCa > 1:32182/1‑69 (MQ=255) tGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCa > 1:2537520/1‑69 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:1574790/47‑1 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:1280128/47‑1 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:2098780/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:525285/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:3478155/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:2719850/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:2057623/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:249619/1‑58 (MQ=255) gATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa > 1:20607/1‑58 (MQ=255) aTCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCaa > 1:370280/1‑68 (MQ=255) tcGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGgt > 1:551748/1‑69 (MQ=255) tcGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGgt > 1:1192822/1‑69 (MQ=255) | GGGAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGT > W3110S.gb/505111‑505244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |