| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 515,934 | C→T | 100% | A43V (GCG→GTG) | ybbM → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 515,934 | 0 | C | T | 91.2% | 89.7 / 1.9 | 34 | A43V (GCG→GTG) | ybbM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (19/12); total (21/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCC > W3110S.gb/515872‑515995 | cTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATa > 1:3309189/1‑67 (MQ=255) ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGgcgagtga > 1:1151065/1‑64 (MQ=255) ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATaa > 1:3611290/1‑67 (MQ=255) cATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat < 1:1052351/68‑1 (MQ=255) aTAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTAGGGCGAGTGATAATTCAGCTGatta < 1:2322460/69‑1 (MQ=255) aaaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:1302554/69‑1 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTg > 1:3407280/1‑69 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc < 1:1300147/67‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc < 1:1642565/67‑1 (MQ=255) aCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc < 1:3184727/67‑1 (MQ=255) tGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAtg < 1:3368142/69‑1 (MQ=255) gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatta > 1:1068924/1‑47 (MQ=255) gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatta > 1:540545/1‑47 (MQ=255) aaaGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtat < 1:2393452/69‑1 (MQ=255) atatTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTatat > 1:1845716/1‑67 (MQ=255) atatTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt > 1:1619503/1‑68 (MQ=255) atatTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt > 1:2819141/1‑68 (MQ=255) atTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc > 1:1858696/1‑48 (MQ=255) atTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTc > 1:2313128/1‑69 (MQ=255) ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc < 1:3259678/47‑1 (MQ=255) ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCa > 1:1992930/1‑69 (MQ=255) ctctGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTg > 1:3138437/1‑54 (MQ=255) ctctGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta > 1:1521616/1‑59 (MQ=255) ctctGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAg < 1:2401454/68‑1 (MQ=255) tGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg > 1:866907/1‑69 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTgg > 1:19692/1‑68 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTgg > 1:3710978/1‑68 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTgg > 1:1109258/1‑68 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:3413547/67‑1 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:3729313/67‑1 (MQ=255) gCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:222020/68‑1 (MQ=255) tCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCATCGTGgatgat > 1:3683398/1‑68 (MQ=255) tCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgatgat > 1:969051/1‑68 (MQ=255) tCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat > 1:1225040/1‑65 (MQ=255) gggCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGcc > 1:3714181/1‑69 (MQ=255) | CTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCC > W3110S.gb/515872‑515995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |