| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 531,446 | C→T | 62.7% | intergenic (‑1/‑229) | ybbS ← / → allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 531,446 | 0 | C | T | 62.7% | 37.9 / 54.3 | 51 | intergenic (‑1/‑229) | ybbS/allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (10/9); new base T (21/11); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
TTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTA > W3110S.gb/531384‑531514 | tttGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAgg < 1:1452909/69‑1 (MQ=255) aCCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGtt > 1:2774060/1‑69 (MQ=255) aCCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGtt > 1:1308277/1‑69 (MQ=255) gCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTc > 1:3395906/1‑39 (MQ=255) gCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTc > 1:2640501/1‑39 (MQ=255) gCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTc > 1:2757911/1‑39 (MQ=255) gCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTc > 1:3542192/1‑39 (MQ=255) aaTGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTg < 1:586106/69‑1 (MQ=255) gAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtt > 1:2841748/1‑69 (MQ=255) gAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtt > 1:2200148/1‑69 (MQ=255) gAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCtttt > 1:3225897/1‑48 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTa < 1:403367/38‑1 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTa < 1:1968583/38‑1 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTa < 1:575408/38‑1 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTa < 1:598080/38‑1 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAttt > 1:1452267/1‑69 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAttt > 1:193745/1‑69 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGt > 1:1113591/1‑64 (MQ=255) aaaGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGt > 1:3387789/1‑64 (MQ=255) aGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtttt > 1:2150342/1‑68 (MQ=255) aGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtttt < 1:3170467/68‑1 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt > 1:66270/1‑69 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt > 1:1841714/1‑69 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt > 1:342578/1‑69 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt > 1:2016518/1‑69 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCt > 1:130660/1‑68 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATACGCAAGTTGCGTGATTTTCtt > 1:2506928/1‑69 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt < 1:2657077/69‑1 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt < 1:748779/69‑1 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt < 1:1570314/69‑1 (MQ=255) ccGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt < 1:2442170/68‑1 (MQ=255) ccGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:3321938/69‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCt > 1:2692193/1‑59 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:374347/1‑69 (MQ=255) tttCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:2755203/1‑69 (MQ=255) ttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:3678457/1‑68 (MQ=255) ttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:262497/1‑68 (MQ=255) ttCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:964025/1‑68 (MQ=255) ttCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:1241859/1‑68 (MQ=255) ttCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:2961711/1‑68 (MQ=255) ttCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGa > 1:2914718/1‑68 (MQ=255) tCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGAtt > 1:1160647/1‑69 (MQ=255) tGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGa < 1:3221539/49‑1 (MQ=255) tCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAg > 1:3295529/1‑69 (MQ=255) tCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAg < 1:3344440/69‑1 (MQ=255) tCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAg < 1:3134796/69‑1 (MQ=255) cGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGt < 1:407332/69‑1 (MQ=255) aaCATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt < 1:1422179/49‑1 (MQ=255) aCATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAt < 1:2485568/68‑1 (MQ=255) aCATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAt < 1:2489940/68‑1 (MQ=255) cATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAt < 1:2006224/67‑1 (MQ=255) cTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTa > 1:1036144/1‑69 (MQ=255) | TTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTA > W3110S.gb/531384‑531514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |