Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 47,828 A→G 76.6% V20V (GTA→GTG kefC → potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb47,8280AG76.6% 126.5 / 36.3 64V20V (GTA→GTGkefCpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (8/7);  new base G (19/30);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTA  >  W3110S.gb/47765‑47889
                                                               |                                                             
catcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCAt                                                          <  1:1032835/69‑1 (MQ=255)
 atcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCAt                                                          <  1:2477763/68‑1 (MQ=255)
   catGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                       >  1:1087480/1‑69 (MQ=255)
   catGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                       >  1:3719502/1‑69 (MQ=255)
    atGGATTGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGccc                                                            <  1:1855244/63‑1 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGccc                                                            <  1:2453077/63‑1 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                       >  1:1770001/1‑68 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGc                                                       >  1:340729/1‑68 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGc                                                       >  1:664719/1‑68 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGCTGATTAATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                       >  1:167501/1‑68 (MQ=255)
    atGGATAGCCATACGCTGATTCAGGCGATGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGc                                                       >  1:2168313/1‑68 (MQ=255)
      ggATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                     <  1:3238052/68‑1 (MQ=255)
      ggATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                     <  1:2360846/68‑1 (MQ=255)
      ggATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                     <  1:294799/68‑1 (MQ=255)
       gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCgg                                                     <  1:1821262/67‑1 (MQ=255)
       gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa                                                   >  1:584020/1‑69 (MQ=255)
       gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa                                                   <  1:617319/69‑1 (MQ=255)
       gATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa                                                   <  1:2981082/69‑1 (MQ=255)
        aTAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa                                                   <  1:2125141/68‑1 (MQ=255)
           gCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTc                                               >  1:1870858/1‑69 (MQ=255)
                aCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                             >  1:77943/1‑50 (MQ=255)
                aCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                             >  1:73707/1‑50 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGccc                                                            >  1:1822736/1‑50 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTc                                               >  1:782936/1‑63 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTc                                         >  1:1992532/1‑69 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTc                                         >  1:2672779/1‑69 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTc                                         >  1:2674189/1‑69 (MQ=255)
                 cGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTc                                         >  1:2889842/1‑69 (MQ=255)
                   cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                       <  1:3394879/69‑1 (MQ=255)
                   cTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTg                                       <  1:3101283/69‑1 (MQ=255)
                       ttCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGAt                                   >  1:3641486/1‑69 (MQ=255)
                           ggCGTTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                <  1:847071/68‑1 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCTCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                <  1:591810/68‑1 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATc                                  >  1:2768553/1‑66 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATc                                  >  1:2593323/1‑66 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATc                                  >  1:2071665/1‑66 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                <  1:2498834/68‑1 (MQ=255)
                           ggCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                >  1:2337150/1‑68 (MQ=255)
                            gcgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt                               <  1:2049656/68‑1 (MQ=255)
                            gcgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt                               <  1:3069563/68‑1 (MQ=255)
                            gcgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt                               <  1:3108268/68‑1 (MQ=255)
                            gcgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt                               <  1:379033/68‑1 (MQ=255)
                             cgcTGATTTATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGt                               <  1:2424241/67‑1 (MQ=255)
                                   tttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACtt                           <  1:2830979/65‑1 (MQ=255)
                                   tttATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACtt                           <  1:3514023/65‑1 (MQ=255)
                                    ttATCTCGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                       >  1:931608/1‑68 (MQ=255)
                                         tcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                  <  1:2062668/68‑1 (MQ=255)
                                         tcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                  <  1:3717440/68‑1 (MQ=255)
                                         tcGGTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTg                  <  1:791138/68‑1 (MQ=255)
                                           ggTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATc               >  1:1472236/1‑69 (MQ=255)
                                           ggTTCGGCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATc               >  1:805861/1‑69 (MQ=255)
                                              tCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCt                       >  1:957321/1‑58 (MQ=255)
                                              tCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTa                      >  1:3062990/1‑59 (MQ=255)
                                                ggCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACtt                           <  1:2727598/52‑1 (MQ=255)
                                                 gCAGCGCTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCgg                                <  1:394178/46‑1 (MQ=255)
                                                      gcTGATTGTGCCCATTGCGGTACGTCTTGGTCTggg                                     <  1:1413794/36‑1 (MQ=255)
                                                      gcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat    <  1:3427099/69‑1 (MQ=255)
                                                      gcTGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCat    <  1:1680317/69‑1 (MQ=255)
                                                       ccgATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatt   <  1:2355300/67‑1 (MQ=255)
                                                        tGATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCatta  <  1:2308224/69‑1 (MQ=255)
                                                         gATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt                   <  1:2729234/51‑1 (MQ=255)
                                                         gATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt                   <  1:2263500/51‑1 (MQ=255)
                                                         gATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt                   <  1:2084682/51‑1 (MQ=255)
                                                         gATTGTGCCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCt                   <  1:1488477/51‑1 (MQ=255)
                                                               |                                                             
CATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTA  >  W3110S.gb/47765‑47889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: