| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 70.1 / NA | 22 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/16); total (6/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAA > W3110S.gb/547642‑547756 | cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTc > 1:2598876/1‑69 (MQ=255) ctggctgtATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc < 1:3420498/69‑1 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:1994752/64‑1 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:2026799/64‑1 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc < 1:186785/69‑1 (MQ=255) tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:3579086/1‑55 (MQ=255) tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:3349067/1‑55 (MQ=255) tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:217410/1‑55 (MQ=255) tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa < 1:2825882/52‑1 (MQ=255) ctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc < 1:422640/65‑1 (MQ=255) ggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg > 1:134203/1‑66 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGCGCAAAACACTGCTTCACGCCGGgccgcc < 1:3141248/59‑1 (MQ=39) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:584183/68‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa < 1:193057/69‑1 (MQ=255) aGCCAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCgg < 1:657546/68‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:2347694/69‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:298126/69‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:1765546/69‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1714016/35‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1044249/35‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGACGGGCCGCCaa > 1:3477393/1‑37 (MQ=37) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:2029447/66‑1 (MQ=255) | CACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAA > W3110S.gb/547642‑547756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |