| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 588,045 | A→G | 27.6% | D711D (GAT→GAC) | nfrA ← | bacteriophage N4 receptor, outer membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 588,045 | 0 | A | G | 27.6% | 40.7 / 17.7 | 29 | D711D (GAT→GAC) | nfrA | bacteriophage N4 receptor, outer membrane subunit |
| Reads supporting (aligned to +/- strand): ref base A (15/6); new base G (6/2); total (21/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
GCAAACGGCGGAAATTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATCCAGACGCTGGTTC > W3110S.gb/587978‑588111 | gCAAACGGCGGAAATTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTa < 1:10309/68‑1 (MQ=255) cAAACGGCGGAAATTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTGTc > 1:1155489/1‑69 (MQ=255) cAAACGGCGGAAATTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATc < 1:1728044/69‑1 (MQ=255) aTTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTGTCAATGTCatcaat > 1:1862278/1‑69 (MQ=255) aTTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTGTCAATGTCatcaat > 1:3678806/1‑69 (MQ=255) gAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCAc < 1:2127124/69‑1 (MQ=255) gAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCAc < 1:2223499/69‑1 (MQ=255) gAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCAc < 1:264905/69‑1 (MQ=255) tgATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATc > 1:345981/1‑44 (MQ=255) gATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTGTCAATGTCATCAATCACCAGCCGGGCg < 1:1376160/68‑1 (MQ=255) aGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGc > 1:1441229/1‑69 (MQ=255) aGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGc > 1:1802353/1‑69 (MQ=255) aGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGACGCAGGc > 1:2304106/1‑69 (MQ=255) aGTGGGGTTATCAGCGCCTGATTATCAATGTCAT‑AATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCa > 1:6198/1‑69 (MQ=255) ggggTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCAt > 1:3238783/1‑68 (MQ=255) gTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCa > 1:1863300/1‑69 (MQ=255) gTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCa > 1:1865302/1‑69 (MQ=255) aTCAGCGCCTGATTGTCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCa > 1:500626/1‑66 (MQ=255) aTCAGCGCCTGATTATCAATGTCATCAATCACCCGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCa > 1:956982/1‑66 (MQ=255) aTCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCa > 1:1129664/1‑66 (MQ=255) cAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTa > 1:2378550/1‑42 (MQ=255) aGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATc > 1:3073303/1‑65 (MQ=255) aGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATc > 1:2522450/1‑65 (MQ=255) aGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATc > 1:3498265/1‑65 (MQ=255) ccTGATTGTCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGc > 1:1762792/1‑49 (MQ=255) ccTGATTGTCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGc < 1:1150093/49‑1 (MQ=255) tGATTGTCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATCCAGACGCTgg > 1:1818664/1‑69 (MQ=255) ttATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATCCAGACGCTGGTTc > 1:227277/1‑69 (MQ=255) tATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGc < 1:2843829/47‑1 (MQ=255) tATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGc < 1:3237341/47‑1 (MQ=255) | GCAAACGGCGGAAATTGAAGCGTTGTTGATTTTGTTCTGGGGTCAGTGGGGTTATCAGCGCCTGATTATCAATGTCATCAATCACCAGCCGGGCGTAGTGCTGCGTCGCAGGCATGTCATCCAGACGCTGGTTC > W3110S.gb/587978‑588111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |