| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 614,426 | C→T | 68.6% | L349L (CTC→CTT) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 614,426 | 0 | C | T | 68.6% | 40.9 / 27.3 | 35 | L349L (CTC→CTT) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (8/3); new base T (11/13); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCAT > W3110S.gb/614359‑614483 | aTGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTc < 1:1530283/68‑1 (MQ=255) gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCaa > 1:31655/1‑68 (MQ=255) gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCaa > 1:2231294/1‑68 (MQ=255) gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAat > 1:2835441/1‑69 (MQ=255) gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAat > 1:639817/1‑69 (MQ=255) gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAat > 1:168296/1‑69 (MQ=255) aaaTTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAgg < 1:2874885/69‑1 (MQ=255) aTTGTCCGTGACAGCGGGCGAGCGGCAGGTTATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTa < 1:595248/69‑1 (MQ=255) tCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTAttt < 1:445080/68‑1 (MQ=255) tGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGAtt > 1:1907591/1‑68 (MQ=255) tGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGAtt > 1:872130/1‑68 (MQ=255) aCAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTAcc < 1:1676866/69‑1 (MQ=255) aGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGatat < 1:2215541/69‑1 (MQ=255) aGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGatat < 1:3589894/69‑1 (MQ=255) gCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCt < 1:2333274/69‑1 (MQ=255) gCAGGTGATGAACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCt < 1:3153402/69‑1 (MQ=255) tgatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg > 1:2263556/1‑68 (MQ=255) gatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg > 1:2402261/1‑67 (MQ=255) gatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg > 1:2936681/1‑67 (MQ=255) gatgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATg > 1:2398176/1‑67 (MQ=255) atgaACCGCTGTTTGGTCCGGTACTTAATATCAAgg < 1:994477/36‑1 (MQ=255) atgaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTc < 1:3425203/69‑1 (MQ=255) gaACCGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAg > 1:3061705/1‑69 (MQ=255) aaCCGCTGTTTGGTCCGGTACTCAATATCAAGGTAttt < 1:1906618/38‑1 (MQ=255) ccGCTGTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGgc > 1:2323206/1‑68 (MQ=255) gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTAGCAACTGGATATTCCTGATGTTCAGGCGCAAAc < 1:2827786/69‑1 (MQ=255) gTTTGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAAc < 1:459629/69‑1 (MQ=255) ttGGTCCGGTACTTAATATCAAGGTATTTGATTACCa > 1:2750791/1‑37 (MQ=255) tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAAccc > 1:3674598/1‑68 (MQ=255) tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAAcc < 1:1091705/67‑1 (MQ=255) tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCa < 1:47348/69‑1 (MQ=255) tGGTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCa > 1:2624001/1‑69 (MQ=255) tGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGa > 1:289288/1‑42 (MQ=255) tGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGa > 1:2031420/1‑42 (MQ=255) tGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCa > 1:2724071/1‑69 (MQ=255) gTCCGGTACTTAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCAt < 1:834295/68‑1 (MQ=255) | ATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCAT > W3110S.gb/614359‑614483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |