| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 810,589 | T→G | 84.1% | M275R (ATG→AGG) | bioB → | biotin synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 810,589 | 0 | T | G | 84.1% | 69.0 / 2.4 | 25 | M275R (ATG→AGG) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base G (8/13); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
AACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGA > W3110S.gb/810530‑810648 | aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTa < 1:127090/68‑1 (MQ=255)aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTa < 1:2146451/68‑1 (MQ=255)aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTa < 1:2604344/68‑1 (MQ=255)aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTAt < 1:3007515/69‑1 (MQ=255) aCGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGgc > 1:2506815/1‑68 (MQ=255) gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCtt > 1:1882143/1‑54 (MQ=255) gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCtt > 1:3664210/1‑54 (MQ=255) cTTTCTGCCGGAGGCTATCAGATGAACGAACAGACTGAGGCGATGTGCtt < 1:3302699/50‑1 (MQ=37) tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTAt > 1:1009114/1‑50 (MQ=255) tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAt > 1:365348/1‑68 (MQ=255) cTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGAttt > 1:256456/1‑69 (MQ=255) ggACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTc < 1:2795513/66‑1 (MQ=255) ggACGCGAGCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTa < 1:825212/68‑1 (MQ=255) cgcgAGCAGATGAACGAACAGACTCAGGCGATGTGCtt < 1:707133/38‑1 (MQ=255) gCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaa < 1:1052613/69‑1 (MQ=255) gCAGATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCaa < 1:371915/69‑1 (MQ=255) gATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAAct > 1:2369794/1‑69 (MQ=255) gATGAACGAACAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAAct > 1:820340/1‑69 (MQ=255) gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTc > 1:474221/1‑45 (MQ=255) gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTc > 1:831657/1‑45 (MQ=255) gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:1630526/62‑1 (MQ=255) gaacgaacAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCa < 1:1485639/62‑1 (MQ=255) cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCAc < 1:2103405/68‑1 (MQ=255) cAGACTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCAc < 1:1789677/68‑1 (MQ=255) cTCAGGCGAGGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGa < 1:2384925/69‑1 (MQ=255) | AACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGA > W3110S.gb/810530‑810648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |