| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 827,597 | C→T | 82.1% | P26P (CCG→CCA) | ybhS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 827,597 | 0 | C | T | 82.1% | 86.2 / 14.3 | 39 | P26P (CCG→CCA) | ybhS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base T (16/16); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
TAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGG > W3110S.gb/827533‑827660 | tAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt > 1:142238/1‑69 (MQ=255)tAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGAt > 1:2282982/1‑69 (MQ=255) aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATc > 1:354442/1‑69 (MQ=255) aaTGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATc > 1:351852/1‑69 (MQ=255) tGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATcg > 1:2592407/1‑68 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATcgcg > 1:2290631/1‑68 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGa > 1:994775/1‑69 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGa > 1:1759235/1‑69 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGa > 1:2313667/1‑69 (MQ=255) ccGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGACAGCTACTTGGATCGCGa > 1:144086/1‑69 (MQ=255) gTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc < 1:1439465/69‑1 (MQ=255) aCCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:529313/68‑1 (MQ=255) aaCAGGAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGtc > 1:698081/1‑69 (MQ=255) cagTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGt > 1:1910333/1‑63 (MQ=255) cagTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCttt < 1:1240602/69‑1 (MQ=255) cagTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCttt > 1:312663/1‑69 (MQ=255) gTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTaa < 1:520224/69‑1 (MQ=255) tAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTaa < 1:69480/68‑1 (MQ=255) agcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:1087398/49‑1 (MQ=255) agcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGa < 1:511295/49‑1 (MQ=255) agcagcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACg < 1:2679172/69‑1 (MQ=255) agcGGGATCACTACCGCGATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca < 1:2610355/68‑1 (MQ=255) agcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGcac < 1:3446070/69‑1 (MQ=255) agcGGGATCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGca < 1:783591/68‑1 (MQ=255) ggATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAgcg > 1:2390593/1‑69 (MQ=255) tCACTACCGCAATCAGCCAGCTACTTGGATCGCGAAc > 1:461553/1‑37 (MQ=255) tCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACg > 1:3637927/1‑38 (MQ=255) tCACTACCGCAATCAGCCAGCTACTTGGATCGCGAACg > 1:2104048/1‑38 (MQ=255) aCCGCAATCAGCCAGCTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAc > 1:268426/1‑68 (MQ=255) gCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTAcgc < 1:587932/67‑1 (MQ=255) tCAGCCAGCTACTTGGATCGCGAACGATCTGCGGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGcc < 1:972728/69‑1 (MQ=255) cagccagcTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACg < 1:2717081/46‑1 (MQ=255) agccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcag > 1:1557295/1‑69 (MQ=255) agccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcag < 1:1481710/69‑1 (MQ=255) agccagcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcag < 1:1477478/69‑1 (MQ=255) cagcTACTTGGATCGCGAACGATCTGCCGCGGCTCTTTAACGCACAGCGCCCGTACGCGACGCcaggac < 1:876528/69‑1 (MQ=255) gcTACTTGGATCGCGAAGGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcaggacag > 1:725489/1‑69 (MQ=255) gcTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcaggacag > 1:2140620/1‑69 (MQ=255) cTACTTGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCcaggacagg < 1:1652243/69‑1 (MQ=255) | TAATGCCGTAACCAAAAATAAACAGCAGTAGCAGCGGGATCACTACCGCAATCAGCCAGCTACTCGGATCGCGAACGATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGG > W3110S.gb/827533‑827660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |