| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 847,104 | A→G | 34.6% | R146R (CGT→CGC) | glnP ← | glutamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 847,104 | 0 | A | G | 34.6% | 26.2 / 22.0 | 28 | R146R (CGT→CGC) | glnP | glutamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (8/10); new base G (5/4); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGC > W3110S.gb/847059‑847166 | gtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAAt > 1:2118545/1‑69 (MQ=255) tttAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGa > 1:2607391/1‑68 (MQ=255) tttAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGa > 1:1321699/1‑68 (MQ=255) tttAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGa > 1:1744755/1‑68 (MQ=255) tttAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGa > 1:1309247/1‑68 (MQ=255) atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATg > 1:1392819/1‑69 (MQ=255) tCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:43647/1‑69 (MQ=255) tCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:2477123/1‑69 (MQ=255) ccACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:1270231/1‑68 (MQ=255) ccACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:3118829/1‑68 (MQ=255) ccACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:1861745/1‑68 (MQ=255) ccACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGttt > 1:386124/1‑68 (MQ=255) ggTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAAc > 1:2595432/1‑69 (MQ=255) aCCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa < 1:2177584/69‑1 (MQ=255) aCCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa < 1:2467843/69‑1 (MQ=255) atggcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTg < 1:1656221/61‑1 (MQ=255) cggcggCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc < 1:156944/69‑1 (MQ=255) cggcggCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc < 1:2836105/69‑1 (MQ=255) cggcggCAGCATCCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc < 1:2398931/69‑1 (MQ=255) ggcggcAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc < 1:1107031/68‑1 (MQ=255) ggcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCg < 1:2898092/69‑1 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGa < 1:3029179/69‑1 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGa < 1:2104926/69‑1 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGa < 1:84074/69‑1 (MQ=255) gcagcaTACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgcg < 1:248826/69‑1 (MQ=255) gcagcaTACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgcg < 1:1502838/69‑1 (MQ=255) cagcaTACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgcgc < 1:3675012/69‑1 (MQ=255) caTGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGAcc < 1:1369572/60‑1 (MQ=255) | GTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGC > W3110S.gb/847059‑847166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |