| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 906,962 | G→A | 100% | H72Y (CAC→TAC) | ybjS ← | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 906,962 | 0 | G | A | 94.7% | 47.9 / ‑3.5 | 19 | H72Y (CAC→TAC) | ybjS | predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (10/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAAC > W3110S.gb/906901‑907010 | cgAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAg > 1:1151529/1‑68 (MQ=255)cgAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAg > 1:2469142/1‑68 (MQ=255)cgAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAg > 1:1900988/1‑68 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:1188697/59‑1 (MQ=255) tCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCcgcg > 1:607430/1‑69 (MQ=255) gAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAg < 1:3376242/69‑1 (MQ=255) gAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAg < 1:1305612/69‑1 (MQ=255) aaGCCTGTTGTGTCCCACAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCcgcg > 1:158316/1‑65 (MQ=255) tgttgtGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCAc > 1:2015383/1‑68 (MQ=255) gtgtCCCCCAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGc < 1:1634407/51‑1 (MQ=255) ccccAGGGTGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTtgt < 1:2435124/69‑1 (MQ=255) ggTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:2268199/35‑1 (MQ=255) ggTGAGGTAAAGCTGGAGCAGTACCACAGCGTATc < 1:3080772/35‑1 (MQ=255) tGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAAc < 1:1683685/69‑1 (MQ=255) tGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGc > 1:328699/1‑46 (MQ=255) tGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGaa > 1:1888968/1‑67 (MQ=255) tGAGGTAAAGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGaa > 1:258018/1‑67 (MQ=255) aaaGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAg > 1:2205539/1‑50 (MQ=255) aaGCTGGAGCAGTACCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGaa < 1:3560382/60‑1 (MQ=255) | CGAACGTTAGCCAGATCGAAAGCCTGTTGTGTCCCCCAGGGTGAGGTAAAGCTGGAGCAGTGCCACAGCGTATCAATGCCCGCGAGCATCACTTTAGCTTGTGATGAAAC > W3110S.gb/906901‑907010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |