| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 913,779 | A→G | 68.1% | L153P (CTG→CCG) | hcp ← | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 913,779 | 0 | A | G | 68.1% | 59.7 / 38.7 | 47 | L153P (CTG→CCG) | hcp | hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases |
| Reads supporting (aligned to +/- strand): ref base A (10/5); new base G (18/14); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
ACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTC > W3110S.gb/913718‑913837 | aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAg > 1:481889/1‑69 (MQ=255)aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAg > 1:485843/1‑69 (MQ=255)aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAg > 1:1867361/1‑69 (MQ=255)aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAg > 1:1909547/1‑69 (MQ=255)aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAg > 1:3395531/1‑69 (MQ=255) aaCATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCa > 1:2927200/1‑62 (MQ=255) aaCATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgag < 1:3403422/69‑1 (MQ=255) aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAAt > 1:3297211/1‑69 (MQ=255) aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAAt > 1:2492769/1‑69 (MQ=255) cgcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:707345/1‑69 (MQ=255) cgcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:3022500/1‑69 (MQ=255) cgcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGtt > 1:2976308/1‑69 (MQ=255) ttCCATATAGGCCGCCTCACCTTTCAGGCCATACAGGCACAGCGGATGCAGGCCGAGAATGTTTTCGcc > 1:22534/1‑69 (MQ=255) ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc > 1:1924822/1‑69 (MQ=255) tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGcc < 1:1942751/68‑1 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATcgcc > 1:2064943/1‑69 (MQ=255) taGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgcc > 1:459107/1‑69 (MQ=255) cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt < 1:1942977/69‑1 (MQ=255) cgccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCttt < 1:2273641/69‑1 (MQ=255) ccgcACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTa < 1:754397/68‑1 (MQ=255) cgcACCTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATc < 1:3153293/69‑1 (MQ=255) gcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt < 1:856009/47‑1 (MQ=255) cTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:392398/69‑1 (MQ=255) cTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:608591/69‑1 (MQ=255) cTTTCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:700193/69‑1 (MQ=255) cTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:68594/69‑1 (MQ=255) ttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa > 1:688626/1‑69 (MQ=255) ttCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa > 1:903143/1‑69 (MQ=255) tCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg > 1:926669/1‑69 (MQ=255) tCAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAg > 1:1750975/1‑69 (MQ=255) cgggCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:2552826/63‑1 (MQ=255) cAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt > 1:213941/1‑65 (MQ=255) cAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:1145596/65‑1 (MQ=255) cAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt > 1:80149/1‑65 (MQ=255) cAGGCCATACAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt < 1:2216148/65‑1 (MQ=255) aCAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTAt > 1:913188/1‑51 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAAt > 1:642242/1‑39 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTa < 1:2477253/64‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTa < 1:915163/64‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtt > 1:2056393/1‑68 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAt < 1:3488343/67‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc < 1:2435653/69‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc < 1:1829976/69‑1 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc > 1:2789228/1‑69 (MQ=255) cAGGCACAGCGGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTc > 1:1624123/1‑69 (MQ=255) cAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtt > 1:469378/1‑68 (MQ=255) cAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtt > 1:2898301/1‑68 (MQ=255) | ACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTC > W3110S.gb/913718‑913837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |