| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 938,015 | A→G | 14.1% | Q75Q (CAA→CAG) | lolA → | chaperone for lipoproteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 938,015 | 0 | A | G | 14.1% | 173.2 / 21.3 | 71 | Q75Q (CAA→CAG) | lolA | chaperone for lipoproteins |
| Reads supporting (aligned to +/- strand): ref base A (35/26); new base G (6/4); total (41/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 | |||||||||||
GAAGGTCAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCAAGC > W3110S.gb/937956‑938083 | gAAGGTCAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAGCCTGATGaa < 1:177515/69‑1 (MQ=255) ggTCAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGc > 1:3635131/1‑69 (MQ=255) gTCAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCa > 1:2894159/1‑69 (MQ=255) cAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCt < 1:2566652/57‑1 (MQ=255) cAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCAtt > 1:2536798/1‑69 (MQ=255) gCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCt > 1:3356729/1‑53 (MQ=255) gATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGtt < 1:2177785/69‑1 (MQ=255) gATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGtt < 1:2789028/69‑1 (MQ=255) cTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt > 1:2906394/1‑69 (MQ=255) ggTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:3618835/64‑1 (MQ=255) ggTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:1187017/64‑1 (MQ=255) gTGAAACGTCCAAACTTATTCAACTTGCATATGACACAACCtgatga < 1:2851702/47‑1 (MQ=255) gTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCtgatga > 1:1084166/1‑47 (MQ=255) gTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCtgatga < 1:2751883/47‑1 (MQ=255) gTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCtgatga < 1:3721466/47‑1 (MQ=255) gTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCtgatga > 1:623947/1‑47 (MQ=255) gTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCtgatga > 1:832424/1‑47 (MQ=255) tGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:82189/62‑1 (MQ=255) tGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:1237424/62‑1 (MQ=255) tGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGt < 1:2211602/68‑1 (MQ=255) gAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTaa > 1:561354/1‑69 (MQ=255) aaaCGTCCAAACTTATTCAACTGGCATATGACACAACCt < 1:3561435/39‑1 (MQ=255) aaCGTCCAAACTTATTCAACTGGCATATGACACAACCtgatg > 1:163680/1‑42 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCt < 1:842228/51‑1 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCt < 1:1893743/51‑1 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCt < 1:2615390/51‑1 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTaaaa < 1:3046947/68‑1 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTaaaa < 1:3535698/68‑1 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAac > 1:3662174/1‑69 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAac > 1:1478397/1‑69 (MQ=255) aCGTCCAAACTTATTCAACTGGCATATGAAACAACCTGATGAAAGCATTCt < 1:1704748/51‑1 (MQ=255) cGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAaca < 1:1671051/69‑1 (MQ=255) gTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAacac > 1:3099859/1‑69 (MQ=255) gTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAacac > 1:2826764/1‑69 (MQ=255) tCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACt > 1:2452314/1‑69 (MQ=255) tCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACt > 1:1767185/1‑69 (MQ=255) tCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACt > 1:1800737/1‑69 (MQ=255) cAAACTTATTCAACTGGCATATGACACAGCCTGATGaa < 1:3189228/38‑1 (MQ=255) cAAACTTATTCAACTGGCATATGACACAGCCTGATGaa < 1:2937441/38‑1 (MQ=255) cAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTaa > 1:1342136/1‑61 (MQ=255) cAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTaa > 1:2954193/1‑61 (MQ=255) aCTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:1936340/50‑1 (MQ=255) aCTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCt < 1:2007996/50‑1 (MQ=255) cTTATTGAACTGGCATATGACACAACCTGATGAAAg > 1:476590/1‑36 (MQ=255) cTTATTCAACTGGCATATGACACAACCTTATGAAAg > 1:1233060/1‑36 (MQ=255) cTTATTCAACTGGCATATGACACAACCTGATGAAAg > 1:1381639/1‑36 (MQ=255) cTTATTCAACTGGCATATGACACAACCTGATGAAAg > 1:3538862/1‑36 (MQ=255) cTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTATAACACTGTGGtt < 1:2124961/69‑1 (MQ=255) cTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGtt < 1:3135860/69‑1 (MQ=255) cTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGtt < 1:3719779/69‑1 (MQ=255) tATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTc > 1:29471/1‑68 (MQ=255) tATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTc > 1:3517046/1‑68 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTTGTTTCTGACGGTAAAaca > 1:3257937/1‑55 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAaca > 1:1871493/1‑55 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAaca > 1:2580754/1‑55 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAaca > 1:1201332/1‑55 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAaca > 1:1469400/1‑55 (MQ=255) cAACTGGCATATGACACAGCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATaa < 1:3693039/69‑1 (MQ=255) cAACTGGCATATGACACAAGCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCta < 1:671194/66‑1 (MQ=255) cAACAGGCATATGACACAGCCTGATGAAAGCATTCt > 1:1894531/1‑36 (MQ=37) tatGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCcgttcg > 1:3392650/1‑68 (MQ=255) atGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACtg < 1:3642443/48‑1 (MQ=255) atGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCcgttcg > 1:3267114/1‑67 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:2623635/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:2518316/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:3656027/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:1997642/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:3613372/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:2741737/1‑69 (MQ=255) tGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:2082342/1‑69 (MQ=255) gACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTg > 1:342328/1‑68 (MQ=255) aaCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCa > 1:3413076/1‑67 (MQ=255) aaCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCa > 1:1247949/1‑67 (MQ=255) aaCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCa > 1:1045748/1‑67 (MQ=255) aCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCAAGc < 1:2451921/69‑1 (MQ=255) aCCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCAAGc < 1:1687330/69‑1 (MQ=255) | GAAGGTCAGGGCGATCTGTGGGTGAAACGTCCAAACTTATTCAACTGGCATATGACACAACCTGATGAAAGCATTCTGGTTTCTGACGGTAAAACACTGTGGTTCTATAACCCGTTCGTTGAGCAAGC > W3110S.gb/937956‑938083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |