| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,016,291 | T→C | 50.0% | V52A (GTA→GCA) | rmf → | ribosome modulation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,016,291 | 0 | T | C | 50.0% | ‑5.7 / 16.9 | 14 | V52A (GTA→GCA) | rmf | ribosome modulation factor |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base C (2/5); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
TATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTT > W3110S.gb/1016227‑1016353 | tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgtagt < 1:1098309/68‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgtagt < 1:1276143/68‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgtagt < 1:2539796/68‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagta < 1:105964/69‑1 (MQ=255)tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagta < 1:2472717/69‑1 (MQ=255) aGACTCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagta < 1:1154915/65‑1 (MQ=255) gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGAtt > 1:2454269/1‑69 (MQ=255) gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGAt > 1:1295281/1‑68 (MQ=255) gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGAt > 1:516370/1‑68 (MQ=255) gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGAt > 1:1718197/1‑68 (MQ=255) cAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGt < 1:13568/69‑1 (MQ=255) aGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGtctc > 1:3037202/1‑69 (MQ=255) ggTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgtagta > 1:3384069/1‑50 (MQ=255) ggTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgtagt > 1:1959733/1‑49 (MQ=255) acaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACATGgcagt < 1:1799421/45‑1 (MQ=255) acaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGgcagt < 1:3664548/45‑1 (MQ=255) gCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGaa < 1:1584493/69‑1 (MQ=255) gggAGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAACGGCCTGATTCTGTCTCTTTAAAAAGAAAcc < 1:781769/69‑1 (MQ=255) aGGCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTc > 1:623419/1‑68 (MQ=255) ggCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCg < 1:1047858/69‑1 (MQ=255) ggCTGGCGAGAAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCg < 1:1474910/69‑1 (MQ=255) agaAGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCgg > 1:1943402/1‑69 (MQ=255) aGCCATGGCGGACAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAgg < 1:2163356/69‑1 (MQ=255) aCAGGGCAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtttt < 1:3053549/69‑1 (MQ=255) | TATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTT > W3110S.gb/1016227‑1016353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |