| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,020,867 | A→G | 57.6% | S159P (TCC→CCC) | sulA ← | SOS cell division inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,020,867 | 0 | A | G | 57.6% | 15.2 / 37.8 | 33 | S159P (TCC→CCC) | sulA | SOS cell division inhibitor |
| Reads supporting (aligned to +/- strand): ref base A (5/9); new base G (6/13); total (11/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
AGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCA > W3110S.gb/1020806‑1020934 | aGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAg > 1:3640513/1‑65 (MQ=255)aGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAg > 1:504044/1‑65 (MQ=255) ggATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGtctc < 1:1108750/65‑1 (MQ=255) ccTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGgaa < 1:3340549/49‑1 (MQ=255) ccTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGagag < 1:2664757/69‑1 (MQ=255) cTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAgg > 1:1815488/1‑69 (MQ=255) aaaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGAt > 1:3022234/1‑69 (MQ=255) aaaTTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGAt > 1:590380/1‑69 (MQ=255) tttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATgc < 1:3192736/68‑1 (MQ=255) ttACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATgc < 1:876881/67‑1 (MQ=255) tACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCt < 1:2565356/69‑1 (MQ=255) ttAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTa < 1:631668/68‑1 (MQ=255) ttAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTa < 1:1756810/68‑1 (MQ=255) aCAAATTAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTgag < 1:3408742/48‑1 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgcg > 1:2394840/1‑55 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgcg > 1:2370913/1‑55 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgcg > 1:2137628/1‑55 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgcg < 1:681825/55‑1 (MQ=255) cAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATgcg > 1:3337261/1‑55 (MQ=255) ttAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:2215232/69‑1 (MQ=255) ttAGAGTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata > 1:3469694/1‑69 (MQ=255) ttAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:3092586/69‑1 (MQ=255) ttAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:1703324/69‑1 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAccc < 1:2904250/69‑1 (MQ=255) gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACAGGACGCATAATAAAccc < 1:2448250/69‑1 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:2547224/61‑1 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:2327838/61‑1 (MQ=255) aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata > 1:2056131/1‑61 (MQ=255) tttttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg < 1:2685977/69‑1 (MQ=255) ttttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg < 1:429739/68‑1 (MQ=255) tttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCa < 1:816015/52‑1 (MQ=255) tttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCa < 1:1185355/52‑1 (MQ=255) attAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCa < 1:2561247/51‑1 (MQ=255) gAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCa < 1:3630328/69‑1 (MQ=255) | AGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCA > W3110S.gb/1020806‑1020934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |