| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,032,491 | C→T | 100% | intergenic (‑357/‑70) | serT ← / → hyaA | tRNA‑Ser/hydrogenase 1, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,032,491 | 0 | C | T | 91.2% | 90.2 / 2.0 | 34 | intergenic (‑357/‑70) | serT/hyaA | tRNA‑Ser/hydrogenase 1, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (25/6); total (28/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACG > W3110S.gb/1032426‑1032554 | cgcCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAg > 1:200994/1‑68 (MQ=255) ccAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGa < 1:925037/69‑1 (MQ=255) cAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGc > 1:3463757/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:101335/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:603754/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:392215/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:3602730/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:2964555/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:2860559/1‑69 (MQ=255) gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCt > 1:1287146/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACg > 1:1074975/1‑61 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTg > 1:721337/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTg > 1:2628462/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTg > 1:2702057/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTg > 1:355386/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTg > 1:1420009/1‑68 (MQ=255) aGGATGAGCTTTCGTTTTCAGCATCGCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCtt > 1:2792203/1‑67 (MQ=255) tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGc < 1:2651626/54‑1 (MQ=255) gTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAgcgc > 1:984223/1‑69 (MQ=255) gTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAgcgc > 1:3408528/1‑69 (MQ=255) gTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAgcgc > 1:306634/1‑69 (MQ=255) tcACGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGc > 1:123041/1‑69 (MQ=255) taaCGTGAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGc > 1:1626412/3‑69 (MQ=255) gAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGgtgt > 1:2588753/1‑69 (MQ=255) gAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGgtgt > 1:100074/1‑69 (MQ=255) gAAGCGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGgtgt > 1:2286682/1‑69 (MQ=255) cGATGGTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCa < 1:403733/69‑1 (MQ=255) gATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCa > 1:1821643/1‑68 (MQ=255) ggTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAagg > 1:93105/1‑68 (MQ=255) gTTTGCCTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAagg > 1:2618201/1‑67 (MQ=255) gTTTGCCTTGCTATAGGGACGTCGCTTGACGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAagg > 1:2751781/1‑67 (MQ=255) cTTGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGgagag < 1:3546966/68‑1 (MQ=255) tGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACg < 1:3627831/69‑1 (MQ=255) tGCTATAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACg < 1:815258/69‑1 (MQ=255) | CGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACG > W3110S.gb/1032426‑1032554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |