| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,068,568 | T→A | 24.0% | G22G (GGT→GGA) | ymdF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,068,568 | 0 | T | A | 24.0% | 44.7 / 12.6 | 25 | G22G (GGT→GGA) | ymdF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/9); new base A (2/4); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGC > W3110S.gb/1068502‑1068630 | aaTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTa > 1:790954/1‑68 (MQ=255) ggCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa < 1:419809/68‑1 (MQ=255) ggCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa < 1:321050/68‑1 (MQ=255) ggCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa < 1:1856713/68‑1 (MQ=255) ggCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa < 1:1075111/68‑1 (MQ=255) ggCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACC‑CGAAAGAGCATCAGAAGCAGGTaaaaaa < 1:3025575/69‑1 (MQ=255) gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaaaa < 1:2048657/69‑1 (MQ=255) gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaaaa > 1:3197140/1‑69 (MQ=255) cATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa < 1:2307882/63‑1 (MQ=255) cATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa < 1:305534/63‑1 (MQ=255) aTCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGac < 1:3627179/69‑1 (MQ=255) ggTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGacagcacagc > 1:154971/1‑69 (MQ=255) tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg > 1:3084143/1‑69 (MQ=255) tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggg > 1:327650/1‑69 (MQ=255) ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCggg > 1:1907659/1‑68 (MQ=255) ttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa > 1:3354395/1‑39 (MQ=255) gCAGAAGACCGCGAAAGAGCATCAGAAGCAGTTaaaaaa < 1:1185250/39‑1 (MQ=255) cAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGTTAATTTCAAg < 1:206647/68‑1 (MQ=255) gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTg > 1:2219936/1‑37 (MQ=255) aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa > 1:1754300/1‑69 (MQ=255) aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:2587521/46‑1 (MQ=255) aGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGc > 1:3325954/1‑69 (MQ=255) aGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGc > 1:392995/1‑69 (MQ=255) aGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGc > 1:575828/1‑69 (MQ=255) ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:2767686/39‑1 (MQ=255) | AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGC > W3110S.gb/1068502‑1068630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |