| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,075,828 | T→C | 67.3% | L1159L (TTA→TTG) | putA ← | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,075,828 | 0 | T | C | 67.3% | 69.1 / 48.3 | 55 | L1159L (TTA→TTG) | putA | fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (7/11); new base C (19/18); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGC > W3110S.gb/1075763‑1075893 | tCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAAc < 1:334838/69‑1 (MQ=255)tCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAAc < 1:3659215/69‑1 (MQ=255)tCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAAc < 1:419469/69‑1 (MQ=255) cACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACg > 1:516366/1‑69 (MQ=255) cccGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGcc > 1:811974/1‑69 (MQ=255) cGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGAttt < 1:1589324/66‑1 (MQ=255) gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:2703745/41‑1 (MQ=255) gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:2480900/41‑1 (MQ=255) gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:2472894/41‑1 (MQ=255) gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACAt < 1:3323301/41‑1 (MQ=255) tgtgTTCCTGCCTGCGCCAGCTCTCCATATTGCGTACACAACTCCTGCAATTCTGGACGATTTGCTGcc > 1:2972626/1‑69 (MQ=255) tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGcc < 1:1809458/69‑1 (MQ=255) tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGcc < 1:2395678/69‑1 (MQ=255) tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGcc < 1:3194582/69‑1 (MQ=255) tgtgTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAAAGCCTGCAATTCTGGACGATTTGCTGcc < 1:1765599/69‑1 (MQ=255) gtgtTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGcc < 1:2404778/68‑1 (MQ=255) gtgtTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGAtt > 1:3247279/1‑61 (MQ=255) gtgtTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGAtt > 1:2980159/1‑61 (MQ=255) gtgtTCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGcc < 1:2269093/68‑1 (MQ=255) ttCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCAt < 1:445457/68‑1 (MQ=255) ttCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCAt < 1:827726/68‑1 (MQ=255) ttCCTGCCTGCGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCAt < 1:1808504/68‑1 (MQ=255) cctgcGCCAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCa > 1:144313/1‑69 (MQ=255) ccAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCa < 1:3129239/68‑1 (MQ=255) ccAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCa < 1:1204397/68‑1 (MQ=255) ccAGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCa < 1:1570346/68‑1 (MQ=255) aGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGc > 1:429147/1‑65 (MQ=255) aGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGc < 1:625728/65‑1 (MQ=255) aGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGc < 1:2346802/65‑1 (MQ=255) aGCTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCAttt < 1:332626/69‑1 (MQ=255) cTCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAg > 1:3288463/1‑69 (MQ=255) tCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCAttt > 1:1670047/1‑66 (MQ=255) tCGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCAttt > 1:2773088/1‑66 (MQ=255) cGCCATATTGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCAttt > 1:1005459/1‑65 (MQ=255) atTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGa > 1:1983832/1‑68 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgctg > 1:2387088/1‑38 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgctg > 1:2962198/1‑38 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgctg > 1:2223869/1‑38 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgctg > 1:650861/1‑38 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtgc > 1:1001718/1‑36 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTtg > 1:733355/1‑35 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGt > 1:3567170/1‑52 (MQ=255) ttGCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGc > 1:351803/1‑54 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:2291318/68‑1 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:201480/68‑1 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:3387483/68‑1 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa > 1:1289278/1‑69 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa < 1:488010/69‑1 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa > 1:2560271/1‑69 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa < 1:2074727/69‑1 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa > 1:3181386/1‑69 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa > 1:2538197/1‑69 (MQ=255) gCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCAGCTGAGTc < 1:3406898/68‑1 (MQ=255) gCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:889223/68‑1 (MQ=255) gCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:2845465/68‑1 (MQ=255) gCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:1155739/68‑1 (MQ=255) gCGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCa > 1:3298366/1‑69 (MQ=255) cGTACACAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTc < 1:291476/67‑1 (MQ=255) aCATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGc > 1:1969121/1‑69 (MQ=255) | TCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGC > W3110S.gb/1075763‑1075893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |