| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,101,111 | G→A | 30.0% | S272L (TCG→TTG) | csgG ← | outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,101,111 | 0 | G | A | 30.0% | 56.6 / 29.8 | 40 | S272L (TCG→TTG) | csgG | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (14/14); new base A (7/5); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
TAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAC > W3110S.gb/1101049‑1101177 | tAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACatat > 1:3152183/1‑69 (MQ=255)tAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACatat > 1:2048670/1‑69 (MQ=255)tAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACatat > 1:370122/1‑69 (MQ=255) aGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCgg < 1:709657/69‑1 (MQ=255) aGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCgg < 1:2337214/69‑1 (MQ=255) tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa < 1:3233668/59‑1 (MQ=255) tAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa < 1:736657/59‑1 (MQ=255) aaaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa < 1:2345315/58‑1 (MQ=255) aaaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTAt < 1:547795/69‑1 (MQ=255) aaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAt > 1:3378560/1‑68 (MQ=255) aaaTGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAt > 1:3568368/1‑68 (MQ=255) tgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATg > 1:485661/1‑51 (MQ=255) tgatAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAAt > 1:829443/1‑69 (MQ=255) tAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTc > 1:1787805/1‑69 (MQ=255) aaTTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCa < 1:993420/69‑1 (MQ=255) ccGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACa < 1:1763164/39‑1 (MQ=255) ccGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCt > 1:2061434/1‑69 (MQ=255) ccGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCAACATATGGCGGTATTTCACCAGAATGTCATTCt > 1:1702395/1‑69 (MQ=255) cTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCg < 1:1532451/69‑1 (MQ=255) tttttATCTGTCAGGATTCCGGTAGAACCAACATATGGCGGTATTTCACCAGAATGt > 1:1733693/1‑57 (MQ=255) cTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCttt > 1:2711881/1‑69 (MQ=255) tCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAtt > 1:1386454/1‑69 (MQ=255) aGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAttt > 1:295912/1‑68 (MQ=255) aGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAttt > 1:3030313/1‑68 (MQ=255) aGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAttt > 1:1435171/1‑68 (MQ=255) aGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAttt > 1:1540888/1‑68 (MQ=255) aGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAtt > 1:822580/1‑67 (MQ=255) aTTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa < 1:3662631/69‑1 (MQ=255) ttCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCaa > 1:456011/1‑69 (MQ=255) ttCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCaa > 1:811405/1‑69 (MQ=255) gTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATcc < 1:422152/68‑1 (MQ=255) gTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATcc < 1:2256089/68‑1 (MQ=255) ggAACCGACATATGGCGGTATTTCA‑CAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCcaca < 1:1228056/69‑1 (MQ=255) gAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCcaca < 1:2223364/69‑1 (MQ=255) gAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCcaca < 1:2943961/69‑1 (MQ=255) aCCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAg < 1:61247/68‑1 (MQ=255) aCCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAg > 1:557963/1‑68 (MQ=255) ccGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc < 1:2299554/69‑1 (MQ=255) ccGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc < 1:2856685/69‑1 (MQ=255) cGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa < 1:1884708/56‑1 (MQ=255) | TAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAC > W3110S.gb/1101049‑1101177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |