| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,109,964 | G→A | 100% | C185C (TGC→TGT) | mdoC ← | membrane protein required for modification of periplasmic glucan |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,109,964 | 0 | G | A | 82.8% | 62.5 / 7.8 | 29 | C185C (TGC→TGT) | mdoC | membrane protein required for modification of periplasmic glucan |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base A (20/4); total (24/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAAT > W3110S.gb/1109901‑1110031 | aCTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATACCCGATGCCGAGACATa > 1:2688945/1‑68 (MQ=255)aCTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACAt > 1:3565119/1‑67 (MQ=255)aCTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATa > 1:2748952/1‑68 (MQ=255) aaTGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAAt < 1:2234160/69‑1 (MQ=255) aTGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATc > 1:3667378/1‑69 (MQ=255) ggCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCAcc < 1:3278982/69‑1 (MQ=255) cGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGa < 1:2206555/69‑1 (MQ=255) cacaATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaa > 1:943814/1‑54 (MQ=255) cacaATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaa > 1:3585840/1‑54 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATaaaaaa > 1:1541333/1‑53 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATaaaaaa > 1:377070/1‑53 (MQ=255) caATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATaaaaaa > 1:2727067/1‑53 (MQ=255) aaaaaTCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCa > 1:3328560/1‑68 (MQ=255) aaaaTCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATaaaaaa > 1:2500007/1‑48 (MQ=255) aaTCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAAt > 1:305976/1‑48 (MQ=255) aaTCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAAt > 1:921128/1‑48 (MQ=255) aTCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCgag > 1:2876808/1‑69 (MQ=255) tCGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGa > 1:260592/1‑52 (MQ=255) cGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGaa > 1:2915719/1‑69 (MQ=255) cGTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGaa > 1:584131/1‑69 (MQ=255) gTTCTTCTTATTACCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAAt > 1:362139/1‑69 (MQ=255) attaCCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTc > 1:1239343/1‑69 (MQ=255) attaCCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTc > 1:2565354/1‑69 (MQ=255) aCCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCgaga > 1:74549/1‑55 (MQ=255) aCCGCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGt > 1:3571933/1‑68 (MQ=255) gCATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGtttt < 1:934147/68‑1 (MQ=255) cATAACCGATGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGtttt > 1:207487/1‑67 (MQ=255) aTGCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAAtt < 1:1778631/68‑1 (MQ=255) gCCGAGACATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAAtttt > 1:1648838/1‑68 (MQ=255) ggCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAat > 1:660219/1‑69 (MQ=255) | ACTCAGAATGGGCGGATACACAATAAAAATCGTTCTTCTTATTACCGCATAACCGATGCCGAGGCATAAAAAAATCACCGATAGTTTTACCATCGAGAATTTTTTATTCGTTTTATCAGAATTTTCTAAAT > W3110S.gb/1109901‑1110031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |