| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,172,268 | T→C | 48.3% | K1092E (AAG→GAG) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,172,268 | 0 | T | C | 48.3% | ‑5.1 / 40.1 | 29 | K1092E (AAG→GAG) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (9/6); new base C (6/8); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGTT > W3110S.gb/1172206‑1172327 | gCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCgg > 1:667295/1‑68 (MQ=255) aTGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTc > 1:1335222/1‑69 (MQ=255) gctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCg > 1:3092/1‑59 (MQ=255) gctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCg > 1:2531076/1‑59 (MQ=255) gctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCg > 1:2052584/1‑59 (MQ=255) gcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:2826545/1‑69 (MQ=255) gcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:3108053/1‑69 (MQ=255) gCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCAc < 1:709255/67‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGcc > 1:3127759/1‑69 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg > 1:2561289/1‑56 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg > 1:3425654/1‑56 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCtt > 1:964283/1‑69 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCt < 1:1714862/68‑1 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCt < 1:2639120/68‑1 (MQ=255) gcagcaAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtc < 1:3246486/68‑1 (MQ=255) agcaAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtctc < 1:1559790/68‑1 (MQ=255) agcaAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtctc < 1:1334485/68‑1 (MQ=255) aaaCCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:3488525/48‑1 (MQ=255) cAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGc > 1:1676330/1‑50 (MQ=255) cAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCACCCCGCCTTtct > 1:2082878/1‑56 (MQ=255) ccAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCtt < 1:2917773/69‑1 (MQ=255) ccAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCtt < 1:234871/69‑1 (MQ=255) ccAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATCACCTTCCAGCtt < 1:59910/69‑1 (MQ=255) cAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCAc < 1:3013320/41‑1 (MQ=255) ggCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCt > 1:145853/1‑69 (MQ=255) tAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCa > 1:2371477/1‑69 (MQ=255) gTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGtt < 1:3351488/68‑1 (MQ=255) gTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGtt < 1:2755109/68‑1 (MQ=255) gTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGtt < 1:3484076/68‑1 (MQ=255) | GCGGTAATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGTT > W3110S.gb/1172206‑1172327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |