| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,175,236 | C→A | 66.6% | Q102H (CAG→CAT) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,175,236 | 0 | C | A | 66.6% | 22.2 / 59.7 | 51 | Q102H (CAG→CAT) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base A (12/22); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACT > W3110S.gb/1175171‑1175304 | tGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGc < 1:2918063/69‑1 (MQ=255)tGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGc < 1:1919938/69‑1 (MQ=255) tgGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGc < 1:518857/67‑1 (MQ=255) ggCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTgg > 1:3556827/1‑68 (MQ=255) gcTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTgg < 1:2259623/69‑1 (MQ=255) gcTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTgg < 1:641317/69‑1 (MQ=255) cTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:1956367/69‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgaggag < 1:1228873/69‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgaggag < 1:1477340/69‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa > 1:1149317/1‑48 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa < 1:3061003/48‑1 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa > 1:515101/1‑48 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa > 1:580988/1‑48 (MQ=255) ttCACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTaa > 1:1522545/1‑48 (MQ=255) cACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGgaga > 1:3648638/1‑68 (MQ=255) cACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGgaga > 1:2488541/1‑68 (MQ=255) cACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:653356/69‑1 (MQ=255) cACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt > 1:29641/1‑69 (MQ=255) cACCGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGAt < 1:2849368/69‑1 (MQ=255) aCCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATa > 1:770408/1‑69 (MQ=255) aCCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATa > 1:2908281/1‑69 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:638866/38‑1 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:640186/38‑1 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:479578/38‑1 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:744106/38‑1 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:904913/38‑1 (MQ=255) ccGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:1953410/38‑1 (MQ=255) cGGAACAATCAGTACGCCACGATGCATCGTCGGTAGc < 1:3325447/37‑1 (MQ=255) cGGAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAAt > 1:204862/1‑69 (MQ=255) ggAACAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATg < 1:900361/69‑1 (MQ=255) aCAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGt > 1:1838571/1‑37 (MQ=255) aCAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTc > 1:600253/1‑68 (MQ=255) aCAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTc > 1:708467/1‑68 (MQ=255) aCAATCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTc > 1:1619696/1‑68 (MQ=255) aTCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtg > 1:950588/1‑68 (MQ=255) tCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgag > 1:586819/1‑53 (MQ=255) tCAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCgag > 1:2126173/1‑53 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:614199/42‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:1805028/42‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:2510895/42‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:1595798/42‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGa < 1:889510/42‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCt < 1:2089628/65‑1 (MQ=255) cAGTACGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCt < 1:826255/65‑1 (MQ=255) tACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAg > 1:951161/1‑68 (MQ=255) aCGCCACCATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAgg < 1:2133059/42‑1 (MQ=38) cGCCACGATGCATCGTCGGTAGCTGGTAAAGGGTGGAAAgg < 1:1821845/41‑1 (MQ=255) ccACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCtgatga < 1:2940602/63‑1 (MQ=255) cACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGaaa < 1:1116816/69‑1 (MQ=255) gCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAAc > 1:2962982/1‑69 (MQ=255) gCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAAc > 1:200272/1‑69 (MQ=255) cTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACt > 1:142318/1‑69 (MQ=255) cTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACt > 1:959989/1‑69 (MQ=255) | TGGAGAAAACTGTGTGGGCAAACGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACT > W3110S.gb/1175171‑1175304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |