| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,181,288 | T→G | 76.7% | D64E (GAT→GAG) | cobB → | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,181,288 | 0 | T | G | 76.7% | 78.9 / 9.0 | 30 | D64E (GAT→GAG) | cobB | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (14/9); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCG > W3110S.gb/1181224‑1181346 | tACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGcc < 1:538743/69‑1 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtg < 1:3172119/69‑1 (MQ=255) gACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCCCAGAGGGCCTGTGGgaag > 1:2647713/1‑68 (MQ=255) gCAGGAATTTCTGCGGAATCAGGTTTTCGTACCTTTCGCGCCGCAGAGGGCCtg < 1:1985158/54‑1 (MQ=255) gCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtg < 1:1085228/54‑1 (MQ=255) aGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCggg > 1:2618187/1‑68 (MQ=255) aaTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGt > 1:3333473/1‑66 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaag > 1:1575591/1‑55 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGt > 1:2847561/1‑65 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGt > 1:1007099/1‑65 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGt > 1:2396940/1‑65 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGt > 1:211127/1‑65 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGt > 1:1189024/1‑65 (MQ=255) aTTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGAGGGAAGAACATCGGGt > 1:1638331/1‑65 (MQ=255) ttCTGCGTAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGa < 1:1038752/69‑1 (MQ=255) ttCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCtg < 1:302323/46‑1 (MQ=255) tCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGAt > 1:3611994/1‑69 (MQ=255) tCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaag < 1:2223795/52‑1 (MQ=255) tCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGAt > 1:342301/1‑69 (MQ=255) gAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc < 1:3482964/68‑1 (MQ=255) gAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc < 1:1306553/68‑1 (MQ=255) aGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:3474960/1‑63 (MQ=255) aTTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa < 1:1993493/51‑1 (MQ=255) aTTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa < 1:762813/51‑1 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGt > 1:3370088/1‑69 (MQ=255) cGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGt > 1:3501482/1‑69 (MQ=255) ccTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCg < 1:3685827/69‑1 (MQ=255) tCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATc > 1:2847585/1‑68 (MQ=255) cgccgcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATcgcg < 1:2938981/68‑1 (MQ=255) cgccgcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATcgcg < 1:855557/68‑1 (MQ=255) | TACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCG > W3110S.gb/1181224‑1181346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |