| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,185,915 | A→G | 39.4% | M36T (ATG→ACG) | potB ← | polyamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,185,915 | 0 | A | G | 39.4% | 18.2 / 35.9 | 33 | M36T (ATG→ACG) | potB | polyamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/6); new base G (9/4); total (23/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTG > W3110S.gb/1185851‑1185974 | gTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCAtttt < 1:1601393/69‑1 (MQ=255) tAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACaaa < 1:2545685/69‑1 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGaaa > 1:1771033/1‑58 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa > 1:104116/1‑61 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa > 1:3230691/1‑61 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa > 1:1896416/1‑61 (MQ=255) tCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACaa > 1:1557657/1‑61 (MQ=255) cAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGcgt > 1:2430549/1‑69 (MQ=255) gagCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTcgcg < 1:2807994/69‑1 (MQ=255) cAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCt < 1:3524721/69‑1 (MQ=255) cAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCt < 1:260232/69‑1 (MQ=255) gtgtAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCa < 1:683983/69‑1 (MQ=255) gtgtAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCa < 1:1314266/69‑1 (MQ=255) gtgtAGTTATCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCa < 1:3233352/69‑1 (MQ=255) gtgtAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCa < 1:43534/69‑1 (MQ=255) gtgtAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCa < 1:3205248/69‑1 (MQ=255) aTCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatc > 1:1198348/1‑67 (MQ=255) aTCCAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatc > 1:1142856/1‑67 (MQ=255) aTCCAGCGTAAAGACCGTTTTCACAAAACTGGCGTCGTCGCGGGTCaaa > 1:2267324/1‑49 (MQ=255) aTCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatc > 1:2429140/1‑67 (MQ=255) aTCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGatc > 1:3018243/1‑67 (MQ=255) ccAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCt > 1:724545/1‑52 (MQ=255) cAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAg > 1:970462/1‑69 (MQ=255) cAGCGTAAAGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAg > 1:1820112/1‑69 (MQ=255) tAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGCTCATCAGGtt > 1:2011576/1‑67 (MQ=255) aaGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:2939345/1‑66 (MQ=255) aaGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:2476125/1‑66 (MQ=255) aaGACCGTTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:1246557/1‑66 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:2588682/1‑66 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:1980651/1‑66 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:1859412/1‑66 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:156438/1‑66 (MQ=255) aaGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTg > 1:1054892/1‑66 (MQ=255) | GTGCAATAGCACTTCAAAATAGAGCGGATCGAGCAGACGCGTGTAGTTATCCAGCGTAAAGACCATTTTGACAAAACTGGCGTCGTCGCGGGTCAAAAAGCTGGTGCCAATGATCATCAGGTTG > W3110S.gb/1185851‑1185974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |