| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,207,066 | A→G | 13.7% | E440E (GAA→GAG) | ymfN → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,207,066 | 0 | A | G | 13.7% | 129.4 / 13.3 | 51 | E440E (GAA→GAG) | ymfN | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base G (3/4); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATT > W3110S.gb/1206998‑1207125 | gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1362391/69‑1 (MQ=255)gAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1596962/69‑1 (MQ=255) aaaCAAAATCTATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:890615/69‑1 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:2935727/69‑1 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga > 1:2567979/1‑69 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga > 1:255458/1‑69 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:1508886/69‑1 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagagga < 1:2009819/69‑1 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga > 1:854344/1‑69 (MQ=255) aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:1768168/69‑1 (MQ=255) aaCAAAATCGCTGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:1811839/1‑69 (MQ=255) aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:921168/1‑69 (MQ=255) aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:2527164/1‑69 (MQ=255) aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:1268613/1‑69 (MQ=255) cAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac < 1:969468/69‑1 (MQ=255) cAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac < 1:2302733/69‑1 (MQ=255) aTCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTg < 1:1073331/69‑1 (MQ=255) tCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGaca > 1:3416288/1‑63 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac > 1:1705844/1‑59 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTg > 1:873829/1‑63 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGAt > 1:2257140/1‑69 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGAt < 1:2300323/69‑1 (MQ=255) gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:1090329/69‑1 (MQ=255) gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:1725077/69‑1 (MQ=255) gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:3622588/69‑1 (MQ=255) gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:930801/68‑1 (MQ=255) gCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:854947/68‑1 (MQ=255) ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTg > 1:2955615/1‑69 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAg < 1:2064427/69‑1 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg > 1:3377311/1‑69 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg > 1:2322285/1‑69 (MQ=255) gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1661574/68‑1 (MQ=255) gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1198254/68‑1 (MQ=255) gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1202550/68‑1 (MQ=255) gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:2518936/68‑1 (MQ=255) gcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:13224/68‑1 (MQ=255) cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:1250534/69‑1 (MQ=255) cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:2628795/69‑1 (MQ=255) cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTcc < 1:295130/69‑1 (MQ=255) gATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTa < 1:3061684/69‑1 (MQ=255) aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga > 1:2743107/1‑41 (MQ=255) aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:240032/41‑1 (MQ=255) aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:2137621/41‑1 (MQ=255) aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga < 1:1181153/41‑1 (MQ=255) aTTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTaa > 1:283312/1‑68 (MQ=255) ggTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg < 1:369372/62‑1 (MQ=255) ggTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg < 1:1636501/62‑1 (MQ=255) ttGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:1997261/46‑1 (MQ=255) gagCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:412626/69‑1 (MQ=255) agCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTa < 1:1115460/69‑1 (MQ=255) tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa < 1:2991795/69‑1 (MQ=255) tGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa < 1:960623/69‑1 (MQ=255) cTGTACGAGAAAGAGGACACTCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:240350/1‑68 (MQ=255) cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:2909583/1‑68 (MQ=255) cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:1590065/1‑68 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAg > 1:3698546/1‑68 (MQ=255) tGTACGCGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:478353/1‑60 (MQ=255) tGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:2852286/1‑60 (MQ=255) tGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt > 1:1139987/1‑60 (MQ=255) gTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt < 1:1641663/38‑1 (MQ=255) cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:70470/1‑69 (MQ=255) cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:395315/1‑69 (MQ=255) cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:928259/1‑69 (MQ=255) cGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt > 1:1905394/1‑69 (MQ=255) cGAGAAAGAAGACACGCTGACTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAt > 1:2583461/1‑68 (MQ=255) agaagaCACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgc < 1:281295/47‑1 (MQ=255) agaagaCACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgc < 1:2860077/47‑1 (MQ=255) | GAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATT > W3110S.gb/1206998‑1207125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |