| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,244,320 | C→T | 100% | C113Y (TGC→TAC) | ldcA ← | L,D‑carboxypeptidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,244,320 | 0 | C | T | 81.8% | 70.5 / 10.8 | 33 | C113Y (TGC→TAC) | ldcA | L,D‑carboxypeptidase A |
| Reads supporting (aligned to +/- strand): ref base C (1/5); new base T (16/11); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGT > W3110S.gb/1244255‑1244388 | cgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCAc < 1:2227155/68‑1 (MQ=255) aGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATc > 1:3681157/1‑68 (MQ=255) aGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATc > 1:3037819/1‑68 (MQ=255) gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc < 1:2781524/69‑1 (MQ=255) gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc < 1:248485/69‑1 (MQ=255) gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc < 1:2411636/69‑1 (MQ=255) gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc < 1:2247809/69‑1 (MQ=255) gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGc > 1:1000290/1‑69 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATg < 1:677587/53‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTAt < 1:977659/69‑1 (MQ=255) tGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTAt < 1:2005062/69‑1 (MQ=255) gggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGt > 1:1066013/1‑69 (MQ=255) gggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGc < 1:3372646/64‑1 (MQ=255) gCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTAt < 1:3201687/65‑1 (MQ=255) cGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTg < 1:2441442/43‑1 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc > 1:845990/1‑41 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGc > 1:1206295/1‑41 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCa > 1:252841/1‑59 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:350855/1‑69 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:1581599/1‑69 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:2515316/1‑69 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:1218882/1‑69 (MQ=255) gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtg > 1:787466/1‑69 (MQ=255) aTGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt < 1:3010140/68‑1 (MQ=255) aTGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt < 1:1389571/68‑1 (MQ=255) cgcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGt > 1:2771395/1‑40 (MQ=255) cgcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGt > 1:2592135/1‑40 (MQ=255) cgcCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGt > 1:1844052/1‑40 (MQ=255) agaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGc > 1:228641/1‑48 (MQ=255) agaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGc > 1:3238325/1‑48 (MQ=255) ccGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:1420046/69‑1 (MQ=255) ccGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:1651725/69‑1 (MQ=255) ccGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc < 1:1149813/69‑1 (MQ=255) cACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTGATGTTGTTGGCGAGCCACCAGt < 1:3385118/69‑1 (MQ=255) | CGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGT > W3110S.gb/1244255‑1244388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |