| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,282,409 | A→G | 15.2% | P323P (CCA→CCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,282,409 | 0 | A | G | 15.2% | 107.3 / 13.4 | 46 | P323P (CCA→CCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (28/11); new base G (5/2); total (33/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
ACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTGG > W3110S.gb/1282344‑1282470 | aCCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc < 1:3290069/69‑1 (MQ=255) gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt < 1:961881/69‑1 (MQ=255) gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt < 1:686438/69‑1 (MQ=255) gCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTc > 1:2658983/1‑69 (MQ=255) gCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTc > 1:2362842/1‑69 (MQ=255) ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTc > 1:716389/1‑64 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGGCAACCCAAGCCAGTATTTCACCGAc > 1:1716144/1‑69 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:1081175/1‑69 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:358848/1‑69 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCg > 1:1659001/1‑68 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc > 1:1230434/1‑69 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc > 1:2280599/1‑69 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:1630083/1‑42 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:3532629/1‑42 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:2997328/1‑42 (MQ=255) ggCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGc > 1:1173750/1‑42 (MQ=255) ccACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTa > 1:39311/1‑69 (MQ=255) ccACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTa > 1:707095/1‑69 (MQ=255) ccACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTa > 1:1273188/1‑69 (MQ=255) tAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACAcc > 1:674754/1‑68 (MQ=255) tAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACAcc > 1:2546879/1‑68 (MQ=255) aaTGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:2399500/69‑1 (MQ=255) aaTGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:92219/69‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCAGCGACTATGTGCGTCGCTACACCGACa < 1:1105164/68‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa < 1:631374/68‑1 (MQ=255) gctgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa < 1:2700384/68‑1 (MQ=255) ctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATg > 1:1862649/1‑69 (MQ=255) tgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:1794888/64‑1 (MQ=255) gcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:2559911/69‑1 (MQ=255) gtaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:380395/3‑59 (MQ=255) gAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:1276778/1‑59 (MQ=255) gAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:1553264/1‑59 (MQ=255) aaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTa > 1:1466791/1‑40 (MQ=255) aaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg > 1:2797995/1‑42 (MQ=255) aaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg > 1:1931463/1‑42 (MQ=255) aaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg > 1:2435311/1‑42 (MQ=255) aaTTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg > 1:2577482/1‑42 (MQ=255) ccACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt > 1:1506408/1‑69 (MQ=255) ccACCTCGACAACCCAAGCCAGAATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt > 1:713416/1‑69 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCt > 1:2545060/1‑46 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTac > 1:1631674/1‑48 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTac < 1:2088276/48‑1 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTac < 1:3045252/48‑1 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTac > 1:2813609/1‑48 (MQ=255) gACAACCCAAGCCAGTATTTCACCGACTATTTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCt > 1:867587/1‑68 (MQ=255) cAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTgg < 1:1668880/68‑1 (MQ=255) | ACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTGG > W3110S.gb/1282344‑1282470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |