| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,290,774 | A→G | 32.0% | intergenic (‑65/‑9) | ychJ ← / → rssA | conserved hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,290,774 | 0 | A | G | 32.0% | 59.6 / 42.4 | 50 | intergenic (‑65/‑9) | ychJ/rssA | conserved hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (8/26); new base G (8/8); total (16/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAA > W3110S.gb/1290711‑1290835 | aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:3442609/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:970717/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:3583150/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:2405310/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:2319666/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:486117/69‑1 (MQ=255)aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:2029009/69‑1 (MQ=255) ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc < 1:2180268/68‑1 (MQ=255) ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCg < 1:120719/69‑1 (MQ=255) ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCg < 1:2554810/69‑1 (MQ=255) ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCg < 1:1527156/69‑1 (MQ=255) ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCg < 1:165464/69‑1 (MQ=255) taatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGa < 1:2592353/69‑1 (MQ=255) taatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGa > 1:734992/1‑69 (MQ=255) ctcCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGAtgt < 1:2030906/68‑1 (MQ=255) tGGAAACAATACCGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGt > 1:2587936/1‑55 (MQ=255) tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGt > 1:2861914/1‑55 (MQ=255) tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGt > 1:407220/1‑55 (MQ=255) tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTAc < 1:712138/69‑1 (MQ=255) tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTAc < 1:750520/69‑1 (MQ=255) tgAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACg < 1:2239057/68‑1 (MQ=255) aaCAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGa < 1:1866139/67‑1 (MQ=255) aTAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCAt > 1:797350/1‑69 (MQ=255) cGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCa > 1:1086047/1‑69 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTc > 1:451696/1‑60 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt > 1:3133100/1‑69 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt > 1:139881/1‑69 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt > 1:853960/1‑69 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt > 1:1091163/1‑69 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTAc < 1:986529/50‑1 (MQ=255) ttAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTAc < 1:2231283/50‑1 (MQ=255) aaCCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGa > 1:1390712/1‑39 (MQ=255) aCCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCtt > 1:2910447/1‑69 (MQ=255) ccGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt < 1:2084337/65‑1 (MQ=255) cGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:3084652/35‑1 (MQ=255) cGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:1240798/35‑1 (MQ=255) cGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:1360956/35‑1 (MQ=255) cGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:547611/35‑1 (MQ=255) cGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:2111712/35‑1 (MQ=255) gCCTTAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCg < 1:118315/69‑1 (MQ=255) gCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCg < 1:550510/69‑1 (MQ=255) tAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaata > 1:1449532/1‑68 (MQ=255) tAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaata < 1:282478/68‑1 (MQ=255) aGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaat < 1:891228/66‑1 (MQ=255) aGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat < 1:64362/69‑1 (MQ=255) tGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATg > 1:687624/1‑63 (MQ=255) tGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATg < 1:3612433/63‑1 (MQ=255) ttAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa > 1:2388041/1‑68 (MQ=255) ccGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaa < 1:569472/68‑1 (MQ=255) ccGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaa < 1:968759/68‑1 (MQ=255) | AATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAA > W3110S.gb/1290711‑1290835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |