| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,339,708 | G→A | 100% | A722T (GCC→ACC) | acnA → | aconitate hydratase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,339,708 | 0 | G | A | 80.7% | 64.0 / 10.5 | 31 | A722T (GCC→ACC) | acnA | aconitate hydratase 1 |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (9/16); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCT > W3110S.gb/1339645‑1339773 | aaaGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAat < 1:1190418/69‑1 (MQ=255) aaGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAata > 1:467718/1‑69 (MQ=255) aaGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAata > 1:1216260/1‑69 (MQ=255) aCTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATAtt > 1:3283124/1‑68 (MQ=255) tAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCAt > 1:555543/1‑69 (MQ=255) aaCTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATc > 1:1605085/1‑69 (MQ=255) tCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGt < 1:234908/69‑1 (MQ=255) tCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGt < 1:2787329/69‑1 (MQ=255) tCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGt < 1:1156283/69‑1 (MQ=255) gTTCGCGGCGTGGTAACCATTAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGa < 1:2132885/67‑1 (MQ=255) gTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGa < 1:2094993/67‑1 (MQ=255) gTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGa < 1:2285279/67‑1 (MQ=255) ttCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAAt < 1:1955030/69‑1 (MQ=255) ttCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAAt > 1:1538307/1‑69 (MQ=255) ttCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAAt < 1:268527/69‑1 (MQ=255) ttCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAAt < 1:3655370/69‑1 (MQ=255) tCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGt > 1:2026376/1‑60 (MQ=255) ggcgTGGTAACCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGt < 1:1267015/67‑1 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGt < 1:1140800/58‑1 (MQ=255) aCCATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGt > 1:810653/1‑67 (MQ=255) cATGAAGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGa < 1:1387190/68‑1 (MQ=255) aaGTGATGATGCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGt < 1:2742093/61‑1 (MQ=255) gatgatGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGAt > 1:2950792/1‑69 (MQ=255) gatgatGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGAt > 1:3538093/1‑69 (MQ=255) gatgatGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGAt > 1:459211/1‑69 (MQ=255) gatgCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGAc > 1:2866239/1‑69 (MQ=255) tgCGCGGCACCTTCACCAATATTCGCATCCGTAATGAAAt < 1:3231536/40‑1 (MQ=255) aCCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCAttt < 1:1130948/68‑1 (MQ=255) ttCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCt > 1:841741/1‑69 (MQ=255) ttCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCt < 1:24813/69‑1 (MQ=255) ttCACCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTGGAAGGGGGGATGACGCGGCATTTAc > 1:3620491/1‑67 (MQ=255) | AAAGACTTTAACTCCTACGGTTCGCGGCGTGGTAACCATGAAGTGATGATGCGCGGCACCTTCGCCAATATTCGCATCCGTAATGAAATGGTGCCTGGCGTTGAAGGGGGGATGACGCGGCATTTACCT > W3110S.gb/1339645‑1339773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |