| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,376,428 | G→A | 100% | V209M (GTG→ATG) | ycjP → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,376,428 | 0 | G | A | 77.2% | 66.0 / 16.1 | 35 | V209M (GTG→ATG) | ycjP | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (17/10); total (25/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCAC > W3110S.gb/1376374‑1376486 | aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGa > 1:2082725/1‑68 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGAc > 1:1241398/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGAc > 1:419465/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGAc > 1:751747/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGAc > 1:3406462/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGAc > 1:2645932/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGAc > 1:236954/1‑69 (MQ=255)aCCGTGCCGTTGGCGATGTCCGGGCTGATATCAGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGAc > 1:2934725/1‑69 (MQ=255) gTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTa > 1:2635968/1‑68 (MQ=255) gTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTa > 1:1933728/1‑68 (MQ=255) gTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTAt > 1:3310263/1‑69 (MQ=255) gTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTAt > 1:1759793/1‑69 (MQ=255) ggCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGc > 1:904003/1‑69 (MQ=255) ggCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGc > 1:734684/1‑69 (MQ=255) ggCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGc > 1:1050337/1‑69 (MQ=255) gATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTc > 1:1035401/1‑69 (MQ=255) gATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTc > 1:616701/1‑69 (MQ=255) gATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTc > 1:3670707/1‑69 (MQ=255) tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGt < 1:2242578/58‑1 (MQ=255) tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGt < 1:810242/58‑1 (MQ=255) tCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGt < 1:452557/58‑1 (MQ=255) tCCGGGCTGATATCCGTGTTCGCCTACTGCTTTATGATGGCGTGGAACGACTATCTGt < 1:3094512/58‑1 (MQ=255) cGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAtt < 1:2234397/67‑1 (MQ=255) cGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAtt < 1:2234480/67‑1 (MQ=255) cggCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAtt < 1:3674658/65‑1 (MQ=255) atatCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGtt > 1:149575/1‑50 (MQ=255) tCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAttt > 1:719537/1‑58 (MQ=255) cGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAgc > 1:1701028/1‑67 (MQ=255) cGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAgc > 1:1212936/1‑67 (MQ=255) ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGc > 1:1425750/1‑69 (MQ=255) cTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAAttt > 1:3078965/1‑69 (MQ=255) cTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCAc > 1:3315200/1‑69 (MQ=255) gCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAAttt < 1:3236665/64‑1 (MQ=255) gCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAAttt < 1:156801/64‑1 (MQ=255) gCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAAttt < 1:1759170/64‑1 (MQ=255) gtggCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCAc < 1:1852350/59‑1 (MQ=255) | ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCAC > W3110S.gb/1376374‑1376486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |