| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,400,571 | G→A | 14.6% | L224L (CTG→TTG) | fnr ← | DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,400,571 | 0 | G | A | 14.6% | 106.7 / 10.1 | 41 | L224L (CTG→TTG) | fnr | DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base A (2/4); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA > W3110S.gb/1400503‑1400638 | cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:981172/69‑1 (MQ=255)cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:764486/69‑1 (MQ=255)cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:667937/69‑1 (MQ=255)cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:1017491/69‑1 (MQ=255)cgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAg < 1:2681092/69‑1 (MQ=255) aTGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACAt < 1:3425226/69‑1 (MQ=255) tGACCAGCAAGCTGGGCCAGCTCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATg > 1:1322865/1‑69 (MQ=255) tGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATg > 1:1973793/1‑69 (MQ=255) gCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCtt < 1:2997922/69‑1 (MQ=255) gggCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa < 1:3351645/69‑1 (MQ=255) ccAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc < 1:902/69‑1 (MQ=255) cAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCg < 1:2137654/69‑1 (MQ=255) gcgtCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGAccc < 1:502737/66‑1 (MQ=255) gcATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGAccc < 1:1122667/69‑1 (MQ=255) cATCGTTATTTTCGATTGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa < 1:2305002/60‑1 (MQ=255) tCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGa > 1:2735165/1‑58 (MQ=255) cGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca < 1:1019764/69‑1 (MQ=255) cGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAACATGCCGCTTTTCTGGAAGCGACCcagca < 1:82637/69‑1 (MQ=255) gTTATTTTCGATGGTGATGTATTTACCTTTGATTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagcag > 1:195803/1‑69 (MQ=255) gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc > 1:784977/1‑59 (MQ=255) gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc < 1:3349043/59‑1 (MQ=255) gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGc > 1:2895715/1‑59 (MQ=255) gTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTCGAAGc > 1:2924672/1‑59 (MQ=255) aTTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:2194248/1‑69 (MQ=255) aTTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:3717395/1‑69 (MQ=255) tCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCATCAGACGGCt < 1:2097478/68‑1 (MQ=255) gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg > 1:1505165/1‑57 (MQ=255) gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTg > 1:350076/1‑61 (MQ=255) gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTg > 1:1182696/1‑61 (MQ=255) aTGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCt < 1:2875293/69‑1 (MQ=255) tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:1490445/49‑1 (MQ=255) tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:1083683/49‑1 (MQ=255) tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:2986731/1‑69 (MQ=255) tATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:2185550/1‑69 (MQ=255) aTTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca > 1:48284/1‑48 (MQ=255) aTTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCcagca < 1:2634674/48‑1 (MQ=255) tttACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAcc > 1:1783347/1‑67 (MQ=255) tttACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:683864/69‑1 (MQ=255) tACCTTTGACTGCCAGCATTCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:2291304/67‑1 (MQ=255) tACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGt < 1:96618/67‑1 (MQ=255) tttGACTGCCAACATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGc < 1:1772259/68‑1 (MQ=255) aCTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCa < 1:3010540/67‑1 (MQ=255) tGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTATACCGTCAGGCCCAGAt < 1:73185/68‑1 (MQ=255) gCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATa > 1:2612230/1‑68 (MQ=255) cAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGtt > 1:1290542/1‑69 (MQ=255) aGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTa > 1:2330617/1‑69 (MQ=255) | CGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTA > W3110S.gb/1400503‑1400638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |