| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,426,674 | T→C | 71.2% | D393D (GAT→GAC) | trkG → | potassium transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,426,674 | 0 | T | C | 71.2% | 93.7 / 43.0 | 59 | D393D (GAT→GAC) | trkG | potassium transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/9); new base C (23/19); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATA > W3110S.gb/1426611‑1426737 | aaaTCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:3453314/69‑1 (MQ=255) aaTCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt < 1:734552/68‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATg < 1:2915352/69‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATg < 1:3655926/69‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATg < 1:2258361/69‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATg < 1:3594594/69‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATg < 1:399438/69‑1 (MQ=255) cAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGGGTAATg < 1:1291166/69‑1 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:2510371/1‑60 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:2328821/1‑60 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:501147/1‑60 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:1397418/1‑60 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:630647/1‑60 (MQ=255) ttCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCgtgt > 1:782831/1‑60 (MQ=255) tcATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTg < 1:468666/68‑1 (MQ=255) cccAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATgg < 1:2668316/69‑1 (MQ=255) cccAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATgg < 1:2228652/69‑1 (MQ=255) ccAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGa > 1:2565422/1‑69 (MQ=255) tgttgAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:513786/1‑69 (MQ=255) tgttgAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGtttcttt > 1:1465541/1‑68 (MQ=255) tgttgAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGtttcttt > 1:1374717/1‑68 (MQ=255) tgttgAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGtttcttt > 1:1856900/1‑68 (MQ=255) gAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCgtgt > 1:195154/1‑39 (MQ=255) gAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCgtgt > 1:748226/1‑39 (MQ=255) gtgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGtttctttt > 1:463744/1‑63 (MQ=255) gtgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt < 1:2021141/68‑1 (MQ=255) gtgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt < 1:900888/68‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:3551467/69‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:573028/69‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:1172118/69‑1 (MQ=255) tgtAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta < 1:2094186/69‑1 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttcttt > 1:1299534/1‑66 (MQ=255) tAAATGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTta > 1:1212901/1‑67 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGATCGTGTAAt > 1:1318512/1‑35 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGATCGTGTAAt < 1:2890702/35‑1 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct < 1:1486919/69‑1 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct < 1:3391548/69‑1 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct < 1:3197936/69‑1 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAct > 1:3064383/1‑69 (MQ=255) aaTGTAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATAc > 1:2368093/1‑68 (MQ=255) tAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATActctc > 1:640842/1‑68 (MQ=255) tAGGAGGGAAGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATActctc > 1:636121/1‑68 (MQ=255) aggaggGAAGATAGTTACAGATCGTGTAATGAGGTCTg < 1:1744121/38‑1 (MQ=255) ggAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACgg < 1:2649119/69‑1 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt > 1:2047257/1‑51 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGtttcttttttctt > 1:1742961/1‑51 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACg > 1:103701/1‑66 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGgtg > 1:1582786/1‑69 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGgtg > 1:1571146/1‑69 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGgtg > 1:3323062/1‑69 (MQ=255) aaGATAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGgtg > 1:2614036/1‑69 (MQ=255) tAGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttt > 1:1717234/1‑68 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:1329056/69‑1 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt > 1:2021281/1‑69 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:1298521/69‑1 (MQ=255) aGTTACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGttttt < 1:2420284/69‑1 (MQ=255) tACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:1554168/68‑1 (MQ=255) tACAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTta < 1:2484533/68‑1 (MQ=255) aCAGACCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTtata > 1:3325738/1‑69 (MQ=255) | AAATCAGCTTTCTCATCCCAGAGCGTTGTTGAGTGTAAATGTAGGAGGGAAGATAGTTACAGATCGTGTAATGAGGTCTGTATGGAGTTTCTTTTTTCTTTATACTCTCTTCACGGTGTTTTTTATA > W3110S.gb/1426611‑1426737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |