| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,427,305 | T→C | 34.8% | V72A (GTT→GCT) | ynaK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,427,305 | 0 | T | C | 34.8% | 18.7 / 19.4 | 23 | V72A (GTT→GCT) | ynaK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/4); new base C (2/6); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGT > W3110S.gb/1427248‑1427362 | aaaTTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTg < 1:455968/69‑1 (MQ=255)aaaTTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTg < 1:2859582/69‑1 (MQ=255) tGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTg < 1:610267/62‑1 (MQ=255) tCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCt > 1:427404/1‑69 (MQ=255) cGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTGt < 1:2715069/50‑1 (MQ=255) cGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTGt < 1:863/50‑1 (MQ=255) cGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTGt < 1:767935/50‑1 (MQ=255) gtgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCAtt < 1:2638199/47‑1 (MQ=255) gtgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCAtt < 1:3397284/47‑1 (MQ=255) gtgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCAtt < 1:541129/47‑1 (MQ=255) gtgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACg > 1:1318738/1‑65 (MQ=255) gtgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACg > 1:747153/1‑65 (MQ=255) tgtTATGGCGGCTGAAATGCTCAAAATGGATTCTGCTCCGGTCATTGTTCTGTCTGGCCTGACGGATg > 1:1322525/1‑68 (MQ=255) aTGCTCAAAATGGATTCTGTTCCGGTCATTGTtctg > 1:2402791/1‑36 (MQ=255) aTGCTCAAAATGGATTCTGTTCCGGTCATTGTtctg > 1:1057406/1‑36 (MQ=255) aTGCTCAAAATGGATTCTGTTCCGGTCATTGTtctg > 1:1816886/1‑36 (MQ=255) aTGCTCAAAATGGATTCTGTTCCGGTCATTGTtctg > 1:1271976/1‑36 (MQ=255) aTGCTCAAAATGGATTCTGTTCCGGTCATTGTtctg > 1:1135350/1‑36 (MQ=255) gCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACg > 1:2606297/1‑69 (MQ=255) cTCAAAATGGATTCTGCTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACg > 1:1571119/1‑68 (MQ=255) aaTGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCa < 1:2161629/67‑1 (MQ=255) aaTGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAg > 1:2886651/1‑68 (MQ=255) aaTGGATTCTGTTCCGGTCATTGTTCTGTCTGGCATGACGGATGAGCAGAAGCAGCGATAACGATCAGt > 1:376980/1‑69 (MQ=255) | AAATTATTGCGGGTCACGGTCGTGTTATGGCGGCTGAAATGCTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGT > W3110S.gb/1427248‑1427362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |