| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,441,198 | G→A | 15.2% | T434I (ACC→ATC) | ydbK ← | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,441,198 | 0 | G | A | 15.2% | 152.9 / 17.1 | 59 | T434I (ACC→ATC) | ydbK | fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (33/17); new base A (4/5); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTT > W3110S.gb/1441134‑1441259 | gTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTcg > 1:105373/1‑68 (MQ=255)gTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTcg > 1:3519877/1‑68 (MQ=255) aaCAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGa > 1:837584/1‑69 (MQ=255) cAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGaaa < 1:2554859/69‑1 (MQ=255) cAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGaaa > 1:2836271/1‑69 (MQ=255) cAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGaaa < 1:3341276/69‑1 (MQ=255) cAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGaaa < 1:1396168/69‑1 (MQ=255) aaaaTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAAc < 1:1490316/69‑1 (MQ=255) aaaaTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAAc < 1:2637613/69‑1 (MQ=255) aaaaTAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAAc < 1:3019951/69‑1 (MQ=255) ccTCTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAAAGCTGCCAt < 1:2187757/69‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACTGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACt > 1:3683372/1‑69 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:516765/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:2556450/62‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:3060997/62‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:327603/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:699090/62‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:1999912/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:692830/62‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:1917897/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc < 1:3605155/62‑1 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:514940/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:1506772/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:574745/1‑62 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACt > 1:3295100/1‑69 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACt > 1:1772071/1‑69 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACt > 1:1642043/1‑69 (MQ=255) tgCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACt > 1:699216/1‑69 (MQ=255) cGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:1029606/63‑1 (MQ=255) cGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCAt < 1:1606324/63‑1 (MQ=255) cGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGATCGCGGa < 1:1952481/52‑1 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGt > 1:1459205/1‑40 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGa > 1:624384/1‑46 (MQ=255) aCGGCGTGGAATTACCGATAATCTTGACATTGTTTTTGGTCGCGGa > 1:556252/1‑46 (MQ=255) gTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGG‑‑ACGCTGCCATCACTACCAAGGCCATaaaa < 1:2997521/69‑1 (MQ=255) ggAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaaaa < 1:10221/69‑1 (MQ=255) gAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATc > 1:2305654/1‑50 (MQ=255) gAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATc > 1:1240471/1‑50 (MQ=255) gAATTACCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATaaa > 1:2216129/1‑67 (MQ=255) gAATTACCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATaaa > 1:2337802/1‑67 (MQ=255) aaTTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACa > 1:1039517/1‑69 (MQ=255) aaTTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACa > 1:2527279/1‑69 (MQ=255) aTTACCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACaa < 1:634260/69‑1 (MQ=255) aTTACCGATAATCTT‑ATATTGTTTCTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACaa < 1:3278533/68‑1 (MQ=255) ttACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTAcc < 1:2300401/53‑1 (MQ=255) ttACCGATAATCTTGATATTGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACaa < 1:894463/68‑1 (MQ=255) aCCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGc > 1:1031374/1‑69 (MQ=255) aaTCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:1828837/1‑35 (MQ=255) aaTCTTGATATTGTTTTTGGTCGCGGAAACGCTGc > 1:2359476/1‑35 (MQ=255) aTCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAg > 1:1088659/1‑68 (MQ=255) aTCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAg > 1:736749/1‑68 (MQ=255) ttGATATTGTTTTTGGTCGCGGAACCGCTGCCATCACTACCAAGGCCATaa > 1:2859344/1‑51 (MQ=255) ttGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaa > 1:1810754/1‑51 (MQ=255) ttGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaa > 1:2266899/1‑51 (MQ=255) ttGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATaa > 1:3175238/1‑51 (MQ=255) ttGTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAg < 1:2337249/69‑1 (MQ=255) gTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGtt > 1:3171873/1‑69 (MQ=255) gTTTTTGATCGCGGAAACGCTGGCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGtt > 1:2701122/1‑69 (MQ=255) gTTTTTGATCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGtt > 1:2841777/1‑69 (MQ=255) | GTAAACAAAATAGCCCTGTGCGTACCACGGCGTGGAATTACCGATAATCTTGATATTGTTTTTGGTCGCGGAAACGCTGCCATCACTACCAAGGCCATAAAACAAGGCTTCCAGTTTCGCCGAGTT > W3110S.gb/1441134‑1441259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |