| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,463,502 | C→T | 73.9% | P299L (CCG→CTG) | paaJ → | predicted beta‑ketoadipyl CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,463,502 | 0 | C | T | 73.9% | 33.4 / 12.4 | 23 | P299L (CCG→CTG) | paaJ | predicted beta‑ketoadipyl CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base T (11/6); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGT > W3110S.gb/1463440‑1463562 | gcgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGcc > 1:175755/1‑69 (MQ=255) tCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGcc > 1:2752277/1‑69 (MQ=255) tCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGcc > 1:973490/1‑69 (MQ=255) gTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCgg > 1:1326910/1‑69 (MQ=255) gCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGaa > 1:3149417/1‑69 (MQ=255) gCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGaa > 1:259654/1‑69 (MQ=255) gCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGa < 1:1353966/68‑1 (MQ=255) aaCCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAAcg > 1:3021995/1‑69 (MQ=255) ggAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAg < 1:425270/69‑1 (MQ=255) ggAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAg < 1:492727/69‑1 (MQ=255) cgcgCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAg > 1:2077749/1‑64 (MQ=255) gcgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTc > 1:2494015/1‑68 (MQ=255) gcgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTc > 1:3402357/1‑68 (MQ=255) gcgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTc > 1:716813/1‑68 (MQ=255) gcgcCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCCCAGGGCTGAGTATTc > 1:2681022/1‑68 (MQ=255) ccTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGa < 1:1726702/69‑1 (MQ=255) ccTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGAGCAGGGCTGAGTATTCACGa < 1:3037848/69‑1 (MQ=255) gggCTTGGTCTGGTGCCTGCAATTCGCCGGGTGCTgg < 1:3468466/37‑1 (MQ=37) gggCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTgg < 1:1431904/37‑1 (MQ=255) gCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:2310522/69‑1 (MQ=255) gCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:1613192/69‑1 (MQ=255) gCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGt < 1:805868/69‑1 (MQ=255) tGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGat > 1:2870424/1‑58 (MQ=255) | GCGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGT > W3110S.gb/1463440‑1463562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |