| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,496,439 | A→G | 10.5% | L116S (TTA→TCA) | ydcI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,496,439 | 0 | A | G | 10.5% | 92.6 / 4.7 | 38 | L116S (TTA→TCA) | ydcI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (18/16); new base G (2/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
TACTCATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAC > W3110S.gb/1496371‑1496496 | tACTCATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTa < 1:2715682/69‑1 (MQ=255) aCTCATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTaa < 1:531795/69‑1 (MQ=255) aCTCATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTaa < 1:3118389/69‑1 (MQ=255) aTTGTCGCAACTTGCAAGGTCTTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATc > 1:2695970/1‑69 (MQ=255) gTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATc > 1:2944228/1‑66 (MQ=255) gTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATc > 1:3228902/1‑66 (MQ=255) tGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAata < 1:3462449/55‑1 (MQ=255) tGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATc < 1:3508137/57‑1 (MQ=255) ggTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgtagg < 1:3586702/69‑1 (MQ=255) gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgtaggt < 1:2730654/69‑1 (MQ=255) gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgtaggt < 1:1343603/69‑1 (MQ=255) gtcgtcTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgtaggt < 1:126754/69‑1 (MQ=255) tctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGc > 1:2273900/1‑68 (MQ=255) tctTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCACAGTGCCGCAGTAGGTAGTGc > 1:1755257/1‑68 (MQ=255) tttttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgt > 1:2547166/1‑57 (MQ=255) tttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgt < 1:1495908/55‑1 (MQ=255) tttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgt < 1:1329673/55‑1 (MQ=255) tttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgt < 1:3626986/55‑1 (MQ=255) tttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCaa > 1:1519498/1‑69 (MQ=255) tttGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCaa > 1:2417578/1‑69 (MQ=255) tgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCt < 1:1764553/68‑1 (MQ=255) gCTGATGAAACTGACCTATAACCGAAGGTGATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCt > 1:93200/1‑67 (MQ=255) gCTGATGAAACTGACCTATAACCGAAGGTGATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTg > 1:362487/1‑68 (MQ=255) tgaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAgt > 1:801498/1‑44 (MQ=255) gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTgacgac < 1:2571539/67‑1 (MQ=255) gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTgacgac < 1:1958222/67‑1 (MQ=255) gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTgac < 1:3135754/64‑1 (MQ=255) gaAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTgac < 1:1247226/64‑1 (MQ=255) aCTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGAcatcat < 1:3069903/69‑1 (MQ=255) cTATAACCGAAGGTGATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCattatt < 1:2117265/67‑1 (MQ=255) cTATAACCGAAGGTGATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCattatt < 1:1584714/67‑1 (MQ=255) ataACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGa > 1:1023206/1‑69 (MQ=255) ataACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGa > 1:350204/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCattatt < 1:3301647/64‑1 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:3514012/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:1742562/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:1313126/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:532558/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:739416/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:2266795/1‑69 (MQ=255) taACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAc > 1:2770033/1‑69 (MQ=255) | TACTCATTGTCGCAACTTGCAAGGTCGTCTCTTTTTGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGAC > W3110S.gb/1496371‑1496496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |