| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,521,063 | C→T | 67.7% | S108F (TCC→TTC) | yncB → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,521,063 | 0 | C | T | 67.7% | 62.6 / 42.4 | 52 | S108F (TCC→TTC) | yncB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (8/8); new base T (24/11); total (33/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTA > W3110S.gb/1520995‑1521130 | gAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATc < 1:346564/69‑1 (MQ=255) aaTCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATc < 1:63749/68‑1 (MQ=255) cTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt < 1:2570832/68‑1 (MQ=255) cTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt < 1:2392153/68‑1 (MQ=255) cTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt < 1:288283/68‑1 (MQ=255) cTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt < 1:1722737/68‑1 (MQ=255) gATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGtgat < 1:1288696/69‑1 (MQ=255) gATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGtgat < 1:2340861/69‑1 (MQ=255) aTCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATc > 1:125341/1‑69 (MQ=255) tGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGaa > 1:704593/1‑69 (MQ=255) tGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGaa > 1:3368312/1‑69 (MQ=255) tGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGaa > 1:1491571/1‑69 (MQ=255) gACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAAc < 1:1837463/67‑1 (MQ=255) aCTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTg > 1:610934/1‑69 (MQ=255) aCTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTg > 1:599475/1‑69 (MQ=255) tGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:3675309/1‑48 (MQ=255) tGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:1684329/1‑48 (MQ=255) ggCTACAGTGGATGGCAAGACTATGACATATTCAGTGgaga > 1:347647/1‑41 (MQ=38) ggCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgat < 1:675247/42‑1 (MQ=255) ggCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgat > 1:2865535/1‑42 (MQ=255) ggCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgat < 1:1010299/42‑1 (MQ=255) cTACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:2920718/1‑37 (MQ=255) cTACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:2268704/1‑37 (MQ=255) cTACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:2849975/1‑37 (MQ=255) cTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCa < 1:296158/69‑1 (MQ=255) cTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCa < 1:2399332/69‑1 (MQ=255) atACAGTGGATGGCAAGACTATGACATATTCAgtggt > 1:575078/2‑37 (MQ=39) tACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCaa < 1:3409420/69‑1 (MQ=255) tACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCaa < 1:747639/69‑1 (MQ=255) tGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGc > 1:1461609/1‑52 (MQ=255) tGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATcc > 1:2852400/1‑69 (MQ=255) tGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATcc > 1:1971074/1‑69 (MQ=255) tGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATcc > 1:597153/1‑69 (MQ=255) tGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATcc > 1:1247628/1‑69 (MQ=255) tGGATGGCAAGACTATGACATATACAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATcc > 1:1054791/1‑69 (MQ=255) gATGGCGAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGatc > 1:2258028/1‑54 (MQ=255) gATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCAt > 1:1308434/1‑69 (MQ=255) gATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCAt > 1:497897/1‑69 (MQ=255) gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCAt > 1:2445296/1‑69 (MQ=255) gATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCAt > 1:2055266/1‑69 (MQ=255) ggCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGt > 1:2382670/1‑69 (MQ=255) ggCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGt > 1:68388/1‑69 (MQ=255) ggCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGat < 1:2826250/50‑1 (MQ=255) ggCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGat < 1:3623258/50‑1 (MQ=255) ggCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGat < 1:1519240/50‑1 (MQ=255) gCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGat < 1:131922/49‑1 (MQ=255) cTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTc > 1:335596/1‑66 (MQ=255) cTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTc > 1:2636922/1‑66 (MQ=255) tGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTc < 1:3307748/63‑1 (MQ=255) tGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTc < 1:711017/63‑1 (MQ=255) aCATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGgtg > 1:2555422/1‑69 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTa > 1:610170/1‑69 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTa > 1:1273775/1‑69 (MQ=255) tCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTa > 1:102806/1‑69 (MQ=255) | GAATCATCCTGATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGCTA > W3110S.gb/1520995‑1521130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |