| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,533,715 | T→C | 26.7% | G62G (GGT→GGC) | ydcC → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,533,715 | 0 | T | C | 26.7% | 41.6 / 18.2 | 30 | G62G (GGT→GGC) | ydcC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/12); new base C (4/4); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
GGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA > W3110S.gb/1533682‑1533781 | ggAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCa < 1:2154510/68‑1 (MQ=35) acacATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCAtt > 1:841081/1‑66 (MQ=35) acaTCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag > 1:47362/1‑69 (MQ=35) acaTCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag > 1:1850355/1‑69 (MQ=35) acaTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag < 1:1680740/69‑1 (MQ=255) acaTCCCGATTTTTTGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag < 1:2123894/69‑1 (MQ=255) ttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTg < 1:758880/68‑1 (MQ=35) ttGAAGCAATATGGTGATTTTGAAAATGGTATTCCGGGTCACGACACCATTGCCAGAGTTGTATCCTg < 1:2869693/68‑1 (MQ=255) tGAAGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGt < 1:3590192/68‑1 (MQ=255) gAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTa < 1:1399746/68‑1 (MQ=35) aaGCAATATGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGt < 1:725470/66‑1 (MQ=255) gCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATcc > 1:794093/1‑61 (MQ=35) gCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAg > 1:1984156/1‑69 (MQ=35) atGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATcc > 1:877007/1‑56 (MQ=255) atGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATcc > 1:753568/1‑56 (MQ=255) atGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCAAGAGTTGTATcc > 1:69315/1‑56 (MQ=11) tGGTGATTTTGAAAATGGTATTCCTTTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTg > 1:1276358/1‑68 (MQ=25) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag < 1:708159/45‑1 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag > 1:995657/1‑45 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag < 1:334484/45‑1 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag > 1:630002/1‑45 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag < 1:3460064/45‑1 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCag > 1:1494038/1‑45 (MQ=38) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTGTCCTGTAt < 1:1534378/60‑1 (MQ=255) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTAt < 1:2935894/60‑1 (MQ=35) tGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTg > 1:1267680/1‑68 (MQ=35) tGGCGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTg > 1:1145632/1‑68 (MQ=25) gTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa < 1:695569/68‑1 (MQ=35) gTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCa < 1:1923367/68‑1 (MQ=35) gTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGACAGAGTTGTATCCTGTATCAGTCCTGCa < 1:2437775/68‑1 (MQ=255) | GGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCA > W3110S.gb/1533682‑1533781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |