| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,581,839 | C→T | 57.7% | M222I (ATG→ATA) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,581,839 | 0 | C | T | 57.7% | 21.1 / 65.3 | 52 | M222I (ATG→ATA) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (11/11); new base T (12/18); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACT > W3110S.gb/1581772‑1581901 | aGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGAc > 1:1865283/1‑68 (MQ=255) gCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACa > 1:432020/1‑68 (MQ=255) cTTTCAAACTTACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:555766/69‑1 (MQ=255) cTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:1638177/69‑1 (MQ=255) cTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGAtata < 1:3434872/69‑1 (MQ=255) gATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGc < 1:411956/69‑1 (MQ=255) gATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGc < 1:1980679/69‑1 (MQ=255) aTGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGcc < 1:1286962/69‑1 (MQ=255) tGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCg > 1:562161/1‑69 (MQ=255) aTCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaa > 1:536281/1‑68 (MQ=255) tggTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:1125080/3‑68 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGt > 1:595082/1‑53 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:2788278/1‑68 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:868099/1‑68 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:569338/1‑68 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaa > 1:3101012/1‑68 (MQ=255) tCGTTTTTAACCCATTGCATAAAAATGGTTGATATAAACTTGCAATAAGCCGt > 1:3305833/1‑53 (MQ=255) cGTTTTTAACCCATTGCATAAAAATGGTTTATATaa < 1:478234/36‑1 (MQ=38) gTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc > 1:2864350/1‑69 (MQ=255) gTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc > 1:457968/1‑69 (MQ=255) tAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAg < 1:2184642/42‑1 (MQ=255) tAACCCATTGCATAAAAATGGTTGATATAAACTTGCCATAAg < 1:614053/42‑1 (MQ=255) aaCCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa < 1:1857149/68‑1 (MQ=255) aTTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGTCACAGAAAAATCAAt < 1:2108742/61‑1 (MQ=255) aTTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt > 1:1735405/1‑61 (MQ=255) aTTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt > 1:865857/1‑61 (MQ=255) aTTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt > 1:2639365/1‑61 (MQ=255) tGCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAt < 1:527989/69‑1 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 1:812526/65‑1 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 1:169943/65‑1 (MQ=255) gCATAAAAATGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 1:291117/65‑1 (MQ=255) gCATAAAAATGGTTGACATAAACTTGCCATAAGGCGTGGGAGGCACAGAAAAATCAATGATACGGAAtg > 1:117652/1‑69 (MQ=255) gCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaa < 1:2900501/51‑1 (MQ=255) gCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtg > 1:1117481/1‑69 (MQ=255) aTAAAAATGGTTGACATAAACTTGCCATAAGCCGTgg < 1:2313229/37‑1 (MQ=255) aaaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt < 1:3133232/69‑1 (MQ=255) aaaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt < 1:2234040/69‑1 (MQ=255) aaaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt < 1:3443955/69‑1 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATAc > 1:972022/1‑58 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtgtg < 1:3006663/66‑1 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:2214719/1‑68 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:13279/1‑68 (MQ=255) aaaaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:1636295/1‑68 (MQ=255) aaaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:195240/1‑68 (MQ=255) aaaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGtt > 1:1880384/1‑68 (MQ=255) aaaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:1338741/69‑1 (MQ=255) aaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 1:2308845/69‑1 (MQ=255) aaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:3555877/68‑1 (MQ=255) aaTGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:3548556/68‑1 (MQ=255) aaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:2214483/68‑1 (MQ=255) aaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:2073548/68‑1 (MQ=255) aaTGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtct < 1:3149745/68‑1 (MQ=255) tGGTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCAc < 1:2502479/69‑1 (MQ=255) ggTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt < 1:159295/69‑1 (MQ=255) ggTTGATATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt < 1:164058/69‑1 (MQ=255) tGATATAAACTTGCCATAAGCCGTGGGAGGCACAGaa < 1:1559042/37‑1 (MQ=255) | AGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACT > W3110S.gb/1581772‑1581901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |