| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,588,010 | T→G | 61.5% | D64A (GAC→GCC) | ydeP ← | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,588,010 | 0 | T | G | 61.5% | 65.5 / 32.8 | 44 | D64A (GAC→GCC) | ydeP | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (8/9); new base G (20/7); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTA > W3110S.gb/1587944‑1588075 | aaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCg < 1:972464/69‑1 (MQ=255)aaagaGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCg < 1:1792554/69‑1 (MQ=255) gagGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAAt < 1:1694467/69‑1 (MQ=255) gagGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAAt < 1:1549631/69‑1 (MQ=255) gagGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAAt < 1:155610/69‑1 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTTCTTTTGCGCCGTTTTCACAAATGGCg < 1:3554129/58‑1 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACt > 1:2006426/1‑69 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCg > 1:1109298/1‑58 (MQ=255) tACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCg < 1:456885/58‑1 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgtg > 1:107941/1‑69 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgtg > 1:672714/1‑69 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgtg > 1:1268463/1‑69 (MQ=255) cTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgtg > 1:1566308/1‑69 (MQ=255) gCTTATCCGTGACTTCCCAGGTGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACt > 1:2753244/1‑64 (MQ=255) gCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACtgtg > 1:2893020/1‑67 (MQ=255) gCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACt > 1:443591/1‑64 (MQ=255) gCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACt > 1:871293/1‑64 (MQ=255) gCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACt > 1:3365272/1‑64 (MQ=255) gCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACt > 1:2324615/1‑64 (MQ=255) tCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGa > 1:657219/1‑69 (MQ=255) ccGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGa < 1:3670046/68‑1 (MQ=255) ccGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGa < 1:1241320/68‑1 (MQ=255) cGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGa < 1:503348/67‑1 (MQ=255) cGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATc > 1:2164812/1‑69 (MQ=255) aCTTCCGAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCg < 1:2179089/42‑1 (MQ=255) aCTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGc < 1:1342995/69‑1 (MQ=255) ggCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACg > 1:273607/1‑41 (MQ=255) ggCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACg > 1:3439138/1‑41 (MQ=255) gCGATTGCTTTTGCGCCGTTTTGACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:2828708/1‑69 (MQ=255) gCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:1319902/1‑69 (MQ=255) gCGATTGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACaa > 1:703538/1‑69 (MQ=255) gATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAgg > 1:2646755/1‑51 (MQ=255) gATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAgg > 1:2186905/1‑51 (MQ=255) gATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAgg > 1:2544457/1‑51 (MQ=255) tGCTTTTGCGCCGTTTTCACAAATGGCGAATGACGCACTGTGCt > 1:1974198/1‑44 (MQ=255) gCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCAc > 1:2665131/1‑37 (MQ=255) gcgcCGTTTTCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTc < 1:2975752/68‑1 (MQ=255) cgcCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCGATGCACAACCCGGACAGTCa < 1:2693043/68‑1 (MQ=255) ttttCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGccc < 1:1122153/69‑1 (MQ=255) ttCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATg > 1:2768837/1‑44 (MQ=255) tCACAAATGGCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCctct > 1:3124197/1‑69 (MQ=255) aaaTGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCt < 1:401369/69‑1 (MQ=255) aTGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTa > 1:1669392/1‑69 (MQ=255) aTGTCGAAAGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTa > 1:1888016/1‑69 (MQ=255) | AAAGAGGCGTTTACCTGCTTATCCGTGACTTCCCAGGCGATTGCTTTTGCGCCGTTTTCACAAATGTCGAATGACGCACTGTGCTTAGGATCTGGCCATGCACAACCCGGACAGTCAAAGCCCTCTGGCTTA > W3110S.gb/1587944‑1588075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |