| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,595,724 | G→A | 44.4% | pseudogene (56/1401 nt) | ydeU ← | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,595,724 | 0 | G | A | 44.4% | 9.7 / 44.4 | 36 | pseudogene (56/1401 nt) | ydeU | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (7/13); new base A (6/10); total (13/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
CTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTG > W3110S.gb/1595660‑1595782 | cTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGatat < 1:1617730/68‑1 (MQ=255) cGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGtt < 1:3176825/67‑1 (MQ=255) cGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGtt < 1:1670775/67‑1 (MQ=255) cGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGtt < 1:649244/67‑1 (MQ=255) cGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGtt < 1:1500771/67‑1 (MQ=255) ggTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGcc > 1:407541/1‑69 (MQ=255) ggTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGcc > 1:2246375/1‑69 (MQ=255) gggCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGtt < 1:2348574/69‑1 (MQ=255) aTTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGt < 1:3344217/69‑1 (MQ=255) aTTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGACATTGTTTCCGATATAGTTGCCGTTAACg > 1:3674785/1‑67 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGatat > 1:2521828/1‑52 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTc < 1:670475/69‑1 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTaa > 1:759346/1‑64 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGta > 1:3136338/1‑68 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc > 1:2590174/1‑69 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc < 1:479407/69‑1 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc < 1:43656/69‑1 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc > 1:2945526/1‑69 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc < 1:3080171/69‑1 (MQ=255) ttATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc > 1:3092205/1‑69 (MQ=255) tATCGCCGCCCAGCGTCTCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTc < 1:2257186/68‑1 (MQ=255) cgccgcCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGt < 1:929879/68‑1 (MQ=255) cgccgcCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGt < 1:1475434/68‑1 (MQ=255) ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtata > 1:602579/1‑47 (MQ=255) ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtata > 1:964842/1‑47 (MQ=255) ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGtata > 1:316036/1‑47 (MQ=255) ccgccCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTT‑CGGtata < 1:2052398/46‑1 (MQ=255) caGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTAt < 1:2787877/68‑1 (MQ=255) caGGCCATTGTTTCCGATATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTAt < 1:1084824/68‑1 (MQ=255) gCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCa < 1:2374423/68‑1 (MQ=255) gCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCa < 1:1078900/68‑1 (MQ=255) aTTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTg < 1:3653196/69‑1 (MQ=255) aTTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTg < 1:810096/69‑1 (MQ=255) aTTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTg < 1:863496/69‑1 (MQ=255) tGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACa < 1:2703396/35‑1 (MQ=255) tGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACa < 1:2623574/35‑1 (MQ=255) tGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGtt > 1:250591/1‑45 (MQ=255) tGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGtt > 1:1902320/1‑45 (MQ=255) | CTTATCGGTGGGCGAATTATCGCCGCCCAGCGTCGCGTTGAACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTG > W3110S.gb/1595660‑1595782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |