| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 488,806 | Δ1 bp | 100% | coding (3047/3363 nt) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 488,806 | 0 | A | . | 77.8% | 59.9 / 22.5 | 27 | coding (3047/3363 nt) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base . (8/13); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATG > W3110S.gb/488745‑488870 | ttGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGt < 1:1658504/71‑1 (MQ=255) gactgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCg > 1:2789525/1‑69 (MQ=255) gactgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGt > 1:303991/1‑70 (MQ=255) gactgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTa > 1:154000/1‑70 (MQ=255) ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGtt < 1:2734158/69‑1 (MQ=255) tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaaaa < 1:1761602/51‑1 (MQ=255) tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaa < 1:443460/49‑1 (MQ=255) tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaa < 1:42400/49‑1 (MQ=255) tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGaa < 1:2196732/49‑1 (MQ=255) cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAGGTGCGTAAAGTGTTACTGaa < 1:985858/69‑1 (MQ=255) gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAggc < 1:2316147/71‑1 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg < 1:520191/69‑1 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACt > 1:2144267/1‑71 (MQ=255) gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACt > 1:532170/1‑71 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTa < 1:547374/45‑1 (MQ=255) aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAg < 1:912424/59‑1 (MQ=255) ccGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTa > 1:2093618/1‑51 (MQ=255) ccGTCTCGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGAGTAAAGTGTTACTGAAGGCGGCGACTGAGCa > 1:2414989/1‑71 (MQ=255) tctcGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGaa < 1:2352840/53‑1 (MQ=255) tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAc > 1:2583525/1‑68 (MQ=255) tcGGCGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc > 1:800407/1‑69 (MQ=255) ggCGTGGCCTATGGCTCCGATCTGG‑AAAAGAGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCa < 1:1159268/68‑1 (MQ=255) cGTGGCCTATGGCTCCGATCTGG‑AAAAGTTCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGt < 1:773569/71‑1 (MQ=255) cGTGGCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGt < 1:1066026/71‑1 (MQ=255) ggCCTATGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGaa < 1:1237162/44‑1 (MQ=39) gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGaa > 1:720829/1‑44 (MQ=255) tGGCTCCGATCTGG‑AAAAGTGCGTAATGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCAc > 1:566265/1‑69 (MQ=255) tGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAg < 1:2159581/59‑1 (MQ=255) tGGCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAg < 1:279366/59‑1 (MQ=255) gCTCCGATCTGG‑AAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAAc > 1:942605/1‑71 (MQ=255) gAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATg < 1:1754738/66‑1 (MQ=255) | TTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATG > W3110S.gb/488745‑488870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |