| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 905,152 | Δ1 bp | 100% | coding (138/324 nt) | ybjQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 905,152 | 0 | T | . | 81.8% | 56.9 / 12.4 | 22 | coding (138/324 nt) | ybjQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base . (10/8); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGG > W3110S.gb/905088‑905211 | cGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGt > 1:1688000/1‑65 (MQ=255) tGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGaaa < 1:126896/71‑1 (MQ=255) gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGaaa > 1:1432981/1‑70 (MQ=255) aaTATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGtc > 1:1724569/1‑51 (MQ=255) aaTATTTTCAGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAg > 1:2229289/1‑69 (MQ=39) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:783128/1‑71 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGaa > 1:865994/1‑67 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGaa > 1:106614/1‑67 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGc < 1:1912114/71‑1 (MQ=255) ttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGaa > 1:1447656/1‑65 (MQ=255) tGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:180272/69‑1 (MQ=255) tGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:1041993/69‑1 (MQ=255) tCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTa < 1:203322/50‑1 (MQ=255) tCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACgg > 1:1171234/1‑58 (MQ=255) tCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGga > 1:2354348/1‑60 (MQ=255) tCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTg < 1:1105212/71‑1 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa > 1:637139/1‑50 (MQ=255) cgATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGa < 1:818013/71‑1 (MQ=255) atatCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAAt < 1:33041/71‑1 (MQ=255) tatCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAAt < 1:385091/70‑1 (MQ=255) tCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTg > 1:1023378/1‑37 (MQ=38) tCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCtt > 1:508279/1‑60 (MQ=255) tCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCtt > 1:575342/1‑60 (MQ=255) gTTGGCGGACG‑TCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAgg > 1:1075557/1‑70 (MQ=255) | CGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGG > W3110S.gb/905088‑905211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |