Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,866,848:1 +T 40.7% coding (562/1140 nt) nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,866,8481.T40.7% 24.0 / 101.9 54coding (562/1140 nt)nlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (20/12);  new base T (14/8);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACC  >  W3110S.gb/2866779‑2866914
                                                                      |                                                                  
cAGAATACGTAATTGTCGGTTGCAACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCt                                                                   >  1:1148487/1‑71 (MQ=255)
 aGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCtt                                                                  >  1:597195/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:2623054/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:263602/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:1580530/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGctct                                                             >  1:1521023/1‑71 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGctct                                                             <  1:399425/70‑1 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTctg                                                            <  1:856038/71‑1 (MQ=255)
       cGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTctg                                                            <  1:478401/71‑1 (MQ=255)
         tAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGct                                                               >  1:1310697/1‑66 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         <  1:2603326/71‑1 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:880719/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:914956/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgctg                                                         >  1:2265367/1‑71 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTctgct                                                          <  1:2225957/70‑1 (MQ=255)
           aTTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCtgctgc                                                        >  1:57879/1‑71 (MQ=255)
            ttGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTctgc                                                           >  1:1654308/1‑67 (MQ=255)
            ttGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACGCCTTGCTctgc                                                           >  1:2553697/1‑67 (MQ=255)
             tGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGt                                                      >  1:1729639/1‑71 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGt                                                      >  1:1384591/1‑71 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCt                                                                   >  1:1732512/1‑57 (MQ=255)
              gTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGt                                                      >  1:933373/1‑70 (MQ=255)
                 ggTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCgg                                                   <  1:700690/71‑1 (MQ=255)
                     gCGACGCAACAGCAACGGTGTAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTg                                               >  1:2052981/1‑71 (MQ=255)
                     gCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGt                                                      >  1:864685/1‑64 (MQ=255)
                     gCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCtttg                                                                >  1:221788/1‑51 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTggg                                             <  1:917987/65‑1 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGc                                      <  1:1128059/71‑1 (MQ=255)
                             aCAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGc                                      <  1:1147352/71‑1 (MQ=255)
                                gCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCAtt                                   >  1:2564855/1‑71 (MQ=255)
                                gCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCAt                                    >  1:2660870/1‑70 (MQ=255)
                                  aaCGGTGGAATTTTGTGCAGGCTTGATCACAACTCCt                                                                   >  1:1470557/1‑37 (MQ=255)
                                   aCGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGt                                            <  1:795552/59‑1 (MQ=255)
                                     ggTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:160570/1‑59 (MQ=255)
                                       tGGAATTTTGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGc                              <  1:2723406/70‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGt                          <  1:1904300/71‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGt                          <  1:2537410/71‑1 (MQ=255)
                                         gAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGTAATGGCATTTCCGCCAGTg                         <  1:2381135/71‑1 (MQ=255)
                                           aTTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGa                        >  1:337196/1‑71 (MQ=255)
                                            ttttGTGCAGGCTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:1642350/1‑53 (MQ=255)
                                            ttttGTGCAGGCTTGATCACAACTCCTTTCCTCTGCTGCGTCGGCCTGGGt                                            >  1:175392/1‑51 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTaa                                          >  1:944543/1‑50 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1113182/1‑59 (MQ=255)
                                              ttGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1346376/1‑59 (MQ=255)
                                                 tgCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCg                  <  1:781871/71‑1 (MQ=255)
                                                       cTTGATCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCg             >  1:1913166/1‑71 (MQ=255)
                                                        ttGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTata                                         >  1:493947/1‑39 (MQ=255)
                                                        ttGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTa                >  1:2728153/1‑66 (MQ=255)
                                                        ttGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTa                >  1:2167257/1‑66 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1662872/71‑1 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1144405/71‑1 (MQ=255)
                                                           aTCACAACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAg         <  1:1143000/71‑1 (MQ=255)
                                                             cacaACTCCTTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGaa          >  1:1654783/1‑68 (MQ=255)
                                                              acaACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGTATTTCCCCAGTGATTGGCGTACCGGAAGCATTa    >  1:2482711/1‑71 (MQ=255)
                                                              acaACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:427030/1‑43 (MQ=255)
                                                              acaACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTc                                 >  1:1219768/1‑43 (MQ=255)
                                                                 aCTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGc        <  1:628368/65‑1 (MQ=255)
                                                                 aCTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTAcc  >  1:1822136/1‑71 (MQ=255)
                                                                  cTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGc        <  1:1176687/64‑1 (MQ=255)
                                                                      |                                                                  
CAGAATACGTAATTGTCGGTTGCGACGCAACAGCAACGGTGGAATTTTGTGCAGGCTTGATCACAACTCCTTGCTCTGCTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACC  >  W3110S.gb/2866779‑2866914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: