| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,235,640:1 | +A | 53.8% | coding (655/1542 nt) | nhaB ← | sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,235,640 | 1 | . | A | 53.8% | 11.5 / 19.6 | 13 | coding (655/1542 nt) | nhaB | sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base A (3/4); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGG > W3110S.gb/1235571‑1235692 | tGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccaca < 1:1176519/71‑2 (MQ=255) cgcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacaca > 1:1261863/1‑68 (MQ=255) cgcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacaca > 1:527033/1‑68 (MQ=255) cgcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacaca > 1:2064394/1‑68 (MQ=255) aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCAt < 1:2300898/71‑1 (MQ=255) agaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACACATGGTCAt > 1:1180203/1‑71 (MQ=255) aaTCGCAAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCATTAcg > 1:2120840/1‑71 (MQ=255) tCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCAt < 1:792196/65‑1 (MQ=255) gCCAAAATGCCAGCCAGCCGCTTTAGCGTTGATCAGGTTCTGTGGTTCGCCCACACATGGTCATTAcgccg > 1:140318/1‑71 (MQ=255) cAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCATTAc < 1:82725/64‑1 (MQ=255) ccagccagCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACACATGGTCATTACGCCGCCTAATGc < 1:164040/70‑1 (MQ=255) ccagccagCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACACATGGTCATTACGCCGCCTAATGc < 1:1515119/70‑1 (MQ=255) cagccGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACACATGGTCATTACGCCGCCTAATGCGGTGcc < 1:1973019/71‑1 (MQ=255) cAGGTTCTGTGGTTCGCCCACACATGGTCATTACGCCGCCTAATGCGGTCCCGaca > 1:2526245/1‑56 (MQ=255) cAGGTTCTGTGGTTCGCCCAC‑CATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatcat > 1:739730/1‑69 (MQ=255) tCTGTGGTTCGCCCAC‑CATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAgg > 1:2659397/1‑68 (MQ=255) gTTCGCCCACACATGGTCATTACGCCGCCTAATGc < 1:2005404/35‑1 (MQ=37) | TGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCAC‑CATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGG > W3110S.gb/1235571‑1235692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |