| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,720,894:1 | +C | 100% | coding (423/1110 nt) | ydhH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,720,894 | 1 | . | C | 87.0% | 61.2 / 3.3 | 23 | coding (423/1110 nt) | ydhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (9/11); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTG > W3110S.gb/1720829‑1720963 | cgtcgCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑ccc > 1:2524251/1‑69 (MQ=255) gCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGc < 1:394432/70‑1 (MQ=255) tCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCa < 1:1128050/70‑1 (MQ=255) cGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCaa > 1:188234/1‑70 (MQ=255) ggTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAata < 1:2655810/70‑1 (MQ=255) gggTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATcgc > 1:166669/1‑70 (MQ=255) ggTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATcgc < 1:2360931/70‑1 (MQ=255) agcagcGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGaa < 1:1950825/71‑1 (MQ=255) atgatgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCaa < 1:1273724/48‑1 (MQ=38) gatgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGaaa < 1:578076/62‑1 (MQ=255) gatgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGaaa < 1:2327838/62‑1 (MQ=255) atgGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGAc > 1:2371837/1‑70 (MQ=255) aCGCAGGTACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGt < 1:1524374/71‑1 (MQ=255) tACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCg > 1:2450002/1‑71 (MQ=255) tACCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCg > 1:1451076/1‑71 (MQ=255) aCCAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTaa > 1:2517959/1‑65 (MQ=255) ccAGCGGTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGgt > 1:753384/1‑71 (MQ=255) ggTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGgtgcgtg < 1:2389571/71‑1 (MQ=255) gTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGaaa < 1:2446520/41‑1 (MQ=38) gTGCGCCTTGTCCTCCCCAAGGCAATATCGCGACGGCGaaa < 1:1334148/41‑1 (MQ=38) cTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTaa > 1:535992/1‑52 (MQ=39) cTTGTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTaa > 1:658370/1‑52 (MQ=39) gTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGa > 1:1938395/1‑40 (MQ=37) gTCCTCCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAAttt < 1:1119176/70‑1 (MQ=255) ct‑ccCAAGGCAATATCGCGACGGCGAAAATCACCGACa < 1:249044/38‑1 (MQ=255) ct‑ccCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTg > 1:1375822/1‑71 (MQ=255) | CGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCT‑CCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTG > W3110S.gb/1720829‑1720963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |