Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 4310365–4311562 4311563 2–1199 39 [0] [0] 41 insH–[alsK] insH,[alsK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 43103640 (0.000)30 (1.110) 15/92 NT 100% intergenic (‑286/‑69) yjcS/insH predicted alkyl sulfatase/IS5 element protein
?W3110S.gb 4311564 = 0 (0.000)coding (916/1029 nt) alsK D‑allose kinase

TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  >  W3110S.gb/4311564‑4311633
                                                                                                                                               
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGAACTACTGTGCCAACGTTTTACTCATGGGGCTTT                                                                          <  1:2645679/71‑1
    TATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTT                                                                           <  1:2620797/66‑1
     ATTGTACCTGTCGTTGGCACAGCTGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                      >  1:1959437/1‑70
        GTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                   <  1:146116/70‑1
        GTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                   >  1:1857181/1‑70
               TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                             <  1:2035297/69‑1
                     GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                      <  1:1467114/70‑1
                     GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                      <  1:953703/70‑1
                       ACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAAC                                                   <  1:2508458/71‑1
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:971624/71‑1
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                >  1:1325421/1‑71
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:1390692/71‑1
                                  TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:2328167/63‑1
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:797185/1‑71
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:1050433/1‑71
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                >  1:1534405/1‑61
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                                 >  1:2380183/1‑44
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTA                                                                         >  1:1994795/1‑36
                                     CGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      <  1:716526/70‑1
                                      GCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                            >  1:65802/1‑63
                                      GCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                            >  1:2515100/1‑63
                                      GCTACTATGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                            >  1:4864/1‑63
                                         ACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:2266127/56‑1
                                          CTGTGCTAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCC                                        >  1:1505111/1‑63
                                          CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGC                                >  1:1307204/1‑71
                                          CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGC                                >  1:1973294/1‑71
                                          CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATT                                  <  1:2465954/69‑1
                                            GTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                              >  1:556990/1‑55
                                            GTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAA                                                    >  1:1878842/1‑49
                                             TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:2255256/52‑1
                                               CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCA                            <  1:2274392/70‑1
                                                  ACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                      >  1:944413/1‑57
                                                     TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCA                            <  1:693278/64‑1
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                              >  1:670631/1‑44
                                                            CATGGTGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCAT               >  1:837776/1‑70
                                                             ATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                                <  1:2347509/36‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG          >  1:2721504/1‑71
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAA           <  1:2219376/70‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAA           <  1:2137748/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA    <  1:1931522/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG      >  1:868249/1‑68
                                                                        GCACAGAACTGTGGCAAAAAACGTTGATGAGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  <  1:1573530/71‑1
                                                                                                                                               
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGA  >  W3110S.gb/4311564‑4311633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.