| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 75,677 | A→C | 100% | F541L (TTT→TTG) | sgrR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 75,677 | 0 | A | C | 82.2% | 78.2 / 4.6 | 28 | F541L (TTT→TTG) | sgrR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base C (16/7); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCC > W3110S.gb/75609‑75744 | ggCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTcaa < 1:207027/71‑1 (MQ=255) ttAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCg < 1:827586/71‑1 (MQ=255) tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGaa < 1:2501991/67‑1 (MQ=255) tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1261796/1‑71 (MQ=255) gtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGa > 1:2317147/1‑64 (MQ=255) aTTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAg > 1:1784749/1‑71 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAggg < 1:448847/70‑1 (MQ=255) ttATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTAtt < 1:2742577/71‑1 (MQ=255) tATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg < 1:1926666/66‑1 (MQ=255) aTCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCa > 1:159133/1‑71 (MQ=255) aaCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATgc > 1:1253796/1‑71 (MQ=255) aaCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATgc > 1:2408301/1‑71 (MQ=255) ccAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCAt < 1:2349495/67‑1 (MQ=255) cAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGc > 1:945931/1‑71 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGa > 1:2334185/1‑49 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGa > 1:2427663/1‑49 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGa > 1:632140/1‑49 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgcg > 1:1348478/1‑71 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgcg > 1:127062/1‑71 (MQ=255) tGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:1918213/71‑1 (MQ=255) tGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:498261/71‑1 (MQ=255) ccGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCa > 1:1241732/1‑51 (MQ=255) tGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAggg < 1:2407012/41‑1 (MQ=255) cccACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:591639/53‑1 (MQ=255) ccACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc > 1:196505/1‑50 (MQ=255) ccACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc > 1:2748562/1‑50 (MQ=255) aCGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAAt > 1:396174/1‑70 (MQ=255) tttCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGcc < 1:1842885/71‑1 (MQ=255) tttAAAATCGAACCAGCCGAGGGTATTCATGCGCa > 1:2687029/1‑35 (MQ=255) | GGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCC > W3110S.gb/75609‑75744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |