| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,237,502 | A→G | 66.7% | K46E (AAA→GAA) | yeiT → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,237,502 | 0 | A | G | 66.7% | 25.2 / 18.5 | 24 | K46E (AAA→GAA) | yeiT | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (8/8); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAA > W3110S.gb/2237443‑2237566 | gTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTa < 1:1186944/66‑1 (MQ=255) tattatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTc > 1:1774905/1‑70 (MQ=255) tattatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTc > 1:380635/1‑70 (MQ=255) tGTCACGACGCTCACTGTAGTCAGGCTTGCCAGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTa < 1:2098067/71‑1 (MQ=255) acgCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGt < 1:2226283/71‑1 (MQ=255) acgCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCg > 1:1782388/1‑70 (MQ=255) gCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTaa < 1:1876746/71‑1 (MQ=255) cTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTa < 1:457914/71‑1 (MQ=255) tAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAg > 1:939290/1‑71 (MQ=255) gTCAGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACt > 1:1613592/1‑54 (MQ=255) tCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCa < 1:2059214/46‑1 (MQ=255) tCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa < 1:14718/67‑1 (MQ=255) aGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTAtt > 1:817141/1‑38 (MQ=255) aGGCTTGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACtt > 1:2630360/1‑52 (MQ=255) ttGCCCGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGcc > 1:2656480/1‑70 (MQ=255) gCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTACCga < 1:2080336/70‑1 (MQ=255) ccGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc < 1:2234115/71‑1 (MQ=255) ccGGCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc < 1:1994680/71‑1 (MQ=255) gCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTa < 1:1895134/52‑1 (MQ=255) gCACAGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTa < 1:1336367/52‑1 (MQ=255) cacaGACCGATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt > 1:1838847/1‑71 (MQ=255) gACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCga > 1:2134595/1‑60 (MQ=255) gATCCGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGaaaa < 1:500216/71‑1 (MQ=255) ccGGGGGAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAaataa > 1:2055696/1‑71 (MQ=255) | GTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAA > W3110S.gb/2237443‑2237566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |